Bordetella pertussis, the causative agent of human whooping cough (pertussis) produces a complex array of virulence factors in order to establish efficient infection in the host. The RNA chaperone Hfq and small regulatory RNAs are key players in posttranscriptional regulation in bacteria and have been shown to play an essential role in virulence of a broad spectrum of bacterial pathogens. This study represents the first attempt to characterize the Hfq regulon of the human pathogen B. pertussis under laboratory conditions as well as upon passage in the host and indicates that loss of Hfq has a profound effect on gene expression in B. pertussis. Comparative transcriptional profiling revealed that Hfq is required for expression of several virulence factors in B. pertussis cells including the Type III secretion system (T3SS). In striking contrast to the wt strain, T3SS did not become operational in the hfq mutant passaged either through mice or macrophages thereby proving that Hfq is required for the functionality of the B. pertussis T3SS. Likewise, expression of virulence factors vag8 and tcfA encoding autotransporter and tracheal colonization factor, respectively, was strongly reduced in the hfq mutant. Importantly, for the first time we demonstrate that B. pertussis T3SS can be activated upon contact with macrophage cells in vitro.

• Klíčová slova
• ABC protein, ATP-binding cassette protein, Bsp22, CFU, colony forming unit, Hfq, OMP, outer membrane protein, P, P-value, RT-qPCR, quantitative reverse transcription polymerase chain reaction, SS medium, Steiner-Scholte medium, T3SS, T3SS, Type III secretion system, infection, transcriptomics, virulence, wt, wild-type,
• MeSH
• bakteriální proteiny genetika   metabolismus   MeSH
• bakteriální RNA genetika   metabolismus   MeSH
• Bordetella pertussis genetika   metabolismus   patogenita   MeSH
• buněčné linie MeSH
• faktory virulence rodu Bordetella genetika   metabolismus   MeSH
• infekce bakteriemi rodu Bordetella mikrobiologie   MeSH
• interakce hostitele a patogenu MeSH
• makrofágy metabolismus   mikrobiologie   MeSH
• myši MeSH
• protein hostitelského faktoru 1 nedostatek   genetika   MeSH
• regulace genové exprese u bakterií * MeSH
• regulon MeSH
• sekreční systém typu III genetika   metabolismus   MeSH
• sekreční systém typu V genetika   metabolismus   MeSH
• stanovení celkové genové exprese MeSH
• transkriptom MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• bakteriální proteiny MeSH
• bakteriální RNA MeSH
• faktory virulence rodu Bordetella MeSH
• protein hostitelského faktoru 1 MeSH
• sekreční systém typu III MeSH
• sekreční systém typu V MeSH
• tracheal colonization factor, Bordetella pertussis MeSH Prohlížeč