Human untranslated region (UTR) databases were searched to identify novel proteins potentially regulated by an iron responsive element (IRE), and found two candidates-cell cycle phosphatase Cdc14A variant 1 and myotonic dystrophy kinase-related Cdc42-binding kinase alpha (MRCKalpha), both possessing a putative IRE in their 3'UTR. In further experiments, we focused on MRCKalpha. Biochemical analyses of the MRCKalpha IRE revealed that it was functional and mediated the response to iron level in the same way as transferrin receptor 1 IREs (TfR) did. Similarly to TfR mRNA, MRCKalpha mRNA is stabilized, when iron supply is low, while it is destabilized under iron-rich conditions. The expression of MRCKalpha mRNA was found to be ubiquitous; the highest levels were noted in testes, the lowest in skeletal muscle. The level of MRCKalpha mRNA in various tissues strongly positively correlates with the level of TfR mRNA, indicating its possible role in the transferrin iron uptake pathway.

• MeSH
• 3' nepřekládaná oblast genetika   MeSH
• buňky K562 MeSH
• CD antigeny metabolismus   MeSH
• DM-kinasa MeSH
• lidé MeSH
• protein-serin-threoninkinasy genetika   metabolismus   MeSH
• proteiny regulující obsah železa genetika   metabolismus   MeSH
• receptory transferinu metabolismus   MeSH
• železo farmakologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 3' nepřekládaná oblast MeSH
• CD antigeny MeSH
• CD71 antigen MeSH Prohlížeč
• CDC42BPA protein, human MeSH Prohlížeč
• DM-kinasa MeSH
• protein-serin-threoninkinasy MeSH
• proteiny regulující obsah železa MeSH
• receptory transferinu MeSH
• železo MeSH