Significantly more beta 2-m is released in MLCs than in control cultures of cells from one donor or in cultures of pooled irradiated cells from several donors. Beta 2-m release increases with cultivation time and depends on the intensity of stimulation. It is highest in three-way MLCs and lowest in one-way MLCs when cells of haploidentical individuals are cocultivated.

• MeSH
• beta-2-mikroglobulin metabolismus   MeSH
• leukocyty metabolismus   MeSH
• lidé MeSH
• test smíšené lymfocytární kultury * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• beta-2-mikroglobulin MeSH

Lymphocytes isolated from peripheral blood of patients with irreversible renal failure and cultivated in non-stimulated cell cultures incorporated significantly more 3H-thymidine and released more beta-2-microglobulin than lymphocytes of the patients' healthy relatives (possible kidney donors) and than lymphocytes of non-related blood donors.

• MeSH
• aktivace lymfocytů MeSH
• chronické selhání ledvin imunologie   MeSH
• lidé MeSH
• lymfocyty imunologie   MeSH
• test smíšené lymfocytární kultury MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH

Immunoprecipitation studies showed that the supernatants from the 4-hour mixed cultures of allogeneic mouse lymphoid cells contained a specific component with the alpha2-globulin electrophoretic mobility which can correspond to, or to which a "lymph node activating factor" can be bound. In addition, during allogeneic cell culture serum proteins with the prealbumin mobility were released into the culture medium and in the control culture of cells from only one donor proteins with the albumin mobility predominated. In the supernatants from the 4-hour cultures of human HLA-identical and HLA-diferent lymphocytes beta2-microglobulin was determined by immunodiffusion and radioimmunologically.

• MeSH
• alfa-globuliny imunologie   MeSH
• antisérum MeSH
• beta-2-mikroglobulin imunologie   MeSH
• chemická precipitace MeSH
• histokompatibilní antigeny izolace a purifikace   MeSH
• HLA antigeny izolace a purifikace   MeSH
• imunodifuze MeSH
• kultivované buňky MeSH
• lymfatické uzliny imunologie   MeSH
• lymfocyty imunologie   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• radioimunoanalýza * MeSH
• tvorba protilátek MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alfa-globuliny MeSH
• antisérum MeSH
• beta-2-mikroglobulin MeSH
• histokompatibilní antigeny MeSH
• HLA antigeny MeSH