In an effort to develop an experimental system suitable for immunological studies in which Bcr-Abl-positive cells are to be used as antigens, we examined the properties of two mouse (Balb/c) established cell lines that express the Bcr-Abl protein and are oncogenic for syngeneic animals. Under standard conditions the two cell lines, viz. Ba-p210 (B210) and 12B1, expressed comparable amounts of the Bcr-Abl protein. However, they differed in a number of characteristics. From the morphological point of view, B210 cells were the more homogeneous, being mainly represented by leukaemic blastic cells with a large number of AgNORs as markers indicating a high proliferative activity. 12B1 cells were more polymorphic and giant cells were detected within their populations. Many 12B1 cells exhibited nuclear segmentation and "band-like" structures. Markers of proliferation were less frequent in 12B1 and the tendency for aging was more pronounced in these cells. The 12B1 cells were slightly more sensitive to imatinib mesylate than B210 cells. In B210 cells, the expression of MHC class I was downregulated, which was not the case with 12B1 cells. Both cell lines induced leukaemia-like disease in mice after intravenous application but, as compared with B210, 12B1 cells were about 100 times more oncogenic and the disease they induced was more aggressive. Moreover, 12B1, but not B210, induced tumours after subcutaneous or intraperitoneal inoculation.

• MeSH
• bcr-abl fúzní proteiny genetika   metabolismus   MeSH
• benzamidy MeSH
• chemorezistence fyziologie   MeSH
• down regulace fyziologie   MeSH
• imatinib mesylát MeSH
• invazivní růst nádoru patofyziologie   MeSH
• leukemie farmakoterapie   metabolismus   patologie   MeSH
• MHC antigeny I. třídy metabolismus   MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• nádorová transformace buněk metabolismus   MeSH
• nádorové biomarkery metabolismus   MeSH
• piperaziny farmakologie   MeSH
• proliferace buněk účinky léků   MeSH
• protinádorové látky farmakologie   MeSH
• pyrimidiny farmakologie   MeSH
• stárnutí buněk fyziologie   MeSH
• transformované buněčné linie metabolismus   patologie   transplantace   MeSH
• transplantace nádorů MeSH
• tvar buňky MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• bcr-abl fúzní proteiny MeSH
• benzamidy MeSH
• imatinib mesylát MeSH
• MHC antigeny I. třídy MeSH
• nádorové biomarkery MeSH
• piperaziny MeSH
• protinádorové látky MeSH
• pyrimidiny MeSH