Mouse polyomavirus (MPyV) is considered a potential tool for the application of gene therapy; however, the current knowledge of the encapsulation of DNA into virions is vague. We used a series of assays based on the encapsidation of a reporter vector into MPyV pseudovirions to identify putative cis-acting elements that are involved in DNA encapsidation. None of the sequences that were derived from MPyV have been shown to solely enhance the encapsidation of a reporter vector in the assay. The frequency of encapsidation strongly correlated with the total intracellular amount of the vector after transfection. The encapsidation of target DNA into the pseudovirions was shown to be non-specific, and the packaging of non-replicated DNA was observed. We propose that the actual concentration of target DNA at the sites of virion formation is the primary factor that determines its selection for encapsidation.

• Klíčová slova
• Assembly, Encapsidation signal, Mouse polyomavirus, Packaging, Pseudovirion,
• MeSH
• buněčné linie MeSH
• genetická terapie přístrojové vybavení   MeSH
• genetické vektory genetika   fyziologie   MeSH
• kapsida metabolismus   MeSH
• lidé MeSH
• myši MeSH
• Polyomavirus genetika   fyziologie   MeSH
• reportérové geny MeSH
• sestavení viru * MeSH
• virion genetika   fyziologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH