OBJECTIVES: To study the balance between the pro-oxidative and antioxidative defence system after repeated painful stimulation in rats and the efficacy of the administration of different antioxidants (vitamins C, E, A, and selenium), analgesics (acetylsalicylic acid, morphine), and their combinations. METHODS: Mechanical clamping of both hind limbs was applied for 10 min for 5 consecutive days in adult male Wistar rats. The tail-flick latency was measured before and after a 5-day nociceptive stimulation with or without the substance application. The reactive oxygen species (ROS) were determined in the sensorimotor cortex. RESULTS: Painful stimulation increased lipoperoxidation which persisted for up to 15 days after it had been discontinued. A simultaneous injection of antioxidants decreased the levels of TBARS, SOD and GSHPx; however, antioxidants applied one week prior to the painful stimulation were ineffective. A simultaneous injection of analgesics reduced stress-induced analgesia caused by the nociceptive stimulation, but did not affect lipoperoxidation. CONCLUSIONS: A combination of antioxidants with analgesics normalized both the oxidative stress and functional (the tail-flick latency) indicators. These results suggest that the administration of antioxidants in pain treatment may be employed to decrease the doses of analgesics and to prevent the negative impact of reactive oxygen species on nociception.

• MeSH
• analgetika farmakologie   MeSH
• antioxidancia farmakologie   MeSH
• Aspirin farmakologie   MeSH
• bolest farmakoterapie   metabolismus   MeSH
• kombinovaná farmakoterapie MeSH
• krysa rodu Rattus MeSH
• kyselina askorbová farmakologie   MeSH
• měření bolesti MeSH
• morfin farmakologie   MeSH
• opioidní analgetika farmakologie   MeSH
• peroxidace lipidů účinky léků   MeSH
• potkani Wistar MeSH
• selen farmakologie   MeSH
• vitamin A farmakologie   MeSH
• vitamin E farmakologie   MeSH
• volné radikály metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• analgetika MeSH
• antioxidancia MeSH
• Aspirin MeSH
• kyselina askorbová MeSH
• morfin MeSH
• opioidní analgetika MeSH
• selen MeSH
• vitamin A MeSH
• vitamin E MeSH
• volné radikály MeSH