Selenium nanoparticles (SeNPs) have recently attracted attention for their antimicrobial and anticancer activities. Nevertheless, their use remains limited due to stability issues. The objective of this study is to investigate the impact of different reaction conditions (including the reducing and stabilizing agents, as well as reaction temperature) on the water dispersion characteristics, stability, and biological activity of SeNPs. The particle characteristics were controlled using sodium borohydride as a strong reducing agent and ascorbic acid as a mild agent. The impact of different stabilizers, namely sodium oleate, quercetin, gelatine, poly(ethyleneimine), and poly(diallyldimethyl-ammonium chloride), was investigated on both particle stability and biological activity. Several destabilizing processes occurred, one of which was continuous reduction to the final Se(-II) oxidation state, which was observed in both synthetic approaches, with using sodium borohydride or ascorbic acid as reducing agents. Non-stabilized SeNP dispersions were stable for a maximum of two weeks, while most stabilized SeNP dispersions remained stable for at least two months, and some remained stable for as long as six months. The antibacterial activity had strong effects, particularly against Gram-positive bacteria, and simultaneously low cytotoxicity against mammalian cells. SeNPs exhibited significant antibacterial efficacy, particularly against Staphylococcus aureus, including methicillin-resistant Staphylococcus aureus strains, even at concentrations as low as 1 mg L-1. SeNPs synthesized utilizing sodium borohydride demonstrate minimal cytotoxicity (EC50 > 100 mg L-1). Interestingly, SeNPs reduced by ascorbic acid demonstrated higher cytotoxicity (EC50 6.8 mg L-1) against the NIH/3T3 cell line. This effect is likely due to the combined cytotoxic effect of SeNPs and ascorbic acid acting as a pro-oxidant at high concentrations.
- MeSH
- antibakteriální látky * chemie farmakologie MeSH
- borohydridy chemie MeSH
- kovové nanočástice * chemie MeSH
- kyselina askorbová chemie MeSH
- lidé MeSH
- methicilin rezistentní Staphylococcus aureus účinky léků MeSH
- mikrobiální testy citlivosti MeSH
- myši MeSH
- nanočástice * chemie MeSH
- oxidace-redukce MeSH
- selen * chemie farmakologie MeSH
- Staphylococcus aureus účinky léků MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky * MeSH
- borohydridy MeSH
- kyselina askorbová MeSH
- selen * MeSH
- sodium borohydride MeSH Prohlížeč
Nanoparticles have drawn significant interest in a range of applications, ranging from biomedical to environmental sciences, due to their distinctive physicochemical characteristics. In this study, it was reported that simple biological production of Ag, Se, and bimetallic Ag2Se nanoparticles (NPs) with Pseudomonas aeruginosa is a promising, low-cost, and environmentally friendly method. For the first time in the scientific literature, Ag2Se nanoparticles have been generated via green bacterial biosynthesis. UV-vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and EDX were used to characterize the produced NPs. Biosynthesized NPs were examined for antibacterial, antibiofilm, and photocatalytic properties, and it was determined that the effects of NPs were dose dependent. The biosynthesized AgNPs, SeNPs, and Ag2Se NPs showed anti-microbial activity against Escherichia coli and Staphylococcus aureus. Minimal inhibitory concentrations (MICs) of E. coli and S. aureus were between 150 and 250 µg/mL. The NPs showed antibiofilm activity against E. coli and S. aureus at sub-MIC levels and reduced biofilm formation by at least 80% at a concentration of 200 µg/mL of each NPs. To photocatalyze the breakdown of Congo red, Ag, Se, and Ag2Se NPs were utilized, and their photocatalytic activity was tested at various concentrations and intervals. A minor decrease of photocatalytic degradation was detected throughout the NPs reuse operation (five cycles). Based on the encouraging findings, the synthesized NPs demonstrated antibacterial, antibiofilm, and photocatalytic properties, suggesting that they might be used in pharmaceutical, medical, environmental, and other applications.
- Klíčová slova
- Ag, Ag2Se nanoparticles, Antibiofilm, Antimicrobial, Photocatalytic, Se,
- MeSH
- antibakteriální látky * farmakologie chemie chemická syntéza MeSH
- biofilmy * účinky léků MeSH
- Escherichia coli * účinky léků MeSH
- katalýza MeSH
- kovové nanočástice * chemie MeSH
- mikrobiální testy citlivosti * MeSH
- Pseudomonas aeruginosa * účinky léků metabolismus MeSH
- selen chemie farmakologie MeSH
- sloučeniny stříbra chemie farmakologie MeSH
- Staphylococcus aureus * účinky léků MeSH
- stříbro * chemie farmakologie metabolismus MeSH
- technologie zelené chemie * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky * MeSH
- selen MeSH
- sloučeniny stříbra MeSH
- stříbro * MeSH
BACKGROUND: Cadmium (Cd) pollution has declined crop yields and quality. Selenium (Se) is a beneficial mineral element that protects plants from oxidative damage, thereby improving crop tolerance to heavy metals. The molecular mechanism of Se-induced Cd tolerance in rice (Oryza sativa) is not yet understood. This study aimed to elucidate the beneficial mechanism of Se (1 mg/kg) in alleviating Cd toxicity in rice seedlings. RESULTS: Exogenous selenium addition significantly improved the toxic effect of cadmium stress on rice seedlings, increasing plant height and fresh weight by 20.53% and 34.48%, respectively, and increasing chlorophyll and carotenoid content by 16.68% and 15.26%, respectively. Moreover, the MDA, ·OH, and protein carbonyl levels induced by cadmium stress were reduced by 47.65%, 67.57%, and 56.43%, respectively. Cell wall metabolism, energy cycling, and enzymatic and non-enzymatic antioxidant systems in rice seedlings were significantly enhanced. Transcriptome analysis showed that the expressions of key functional genes psbQ, psbO, psaG, psaD, atpG, and PetH were significantly up-regulated under low-concentration Se treatment, which enhanced the energy metabolism process of photosystem I and photosystem II in rice seedlings. At the same time, the up-regulation of LHCA, LHCB family, and C4H1, PRX, and atp6 functional genes improved the ability of photon capture and heavy metal ion binding in plants. Combined with proteome analysis, the expression of functional proteins OsGSTF1, OsGSTU11, OsG6PDH4, OsDHAB1, CP29, and CabE was significantly up-regulated under Se, which enhanced photosynthesis and anti-oxidative stress mechanism in rice seedlings. At the same time, it regulates the plant hormone signal transduction pathway. It up-regulates the expression response process of IAA, ABA, and JAZ to activate the synergistic effect between each cell rapidly and jointly maintain the homeostasis balance. CONCLUSION: Our results revealed the regulation process of Se-mediated critical metabolic pathways, functional genes, and proteins in rice under cadmium stress. They provided insights into the expression rules and dynamic response process of the Se-mediated plant resistance mechanism. This study provided the theoretical basis and technical support for crop safety in cropland ecosystems and cadmium-contaminated areas.
- Klíčová slova
- Cd contamination, Oryza sativa L, Proteome, Selenium, Transcriptome,
- MeSH
- fyziologický stres genetika účinky léků MeSH
- kadmium * toxicita MeSH
- proteomika * MeSH
- regulace genové exprese u rostlin účinky léků MeSH
- rostlinné geny MeSH
- rostlinné proteiny * genetika metabolismus MeSH
- rýže (rod) * genetika metabolismus účinky léků MeSH
- selen * farmakologie MeSH
- semenáček * genetika účinky léků metabolismus MeSH
- stanovení celkové genové exprese MeSH
- transkriptom MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kadmium * MeSH
- rostlinné proteiny * MeSH
- selen * MeSH
Selenium is an essential trace element that is crucial for cellular antioxidant defense against reactive oxygen species (ROS). Recently, many selenium-containing compounds have exhibited a wide spectrum of biological activities that make them promising scaffolds in Medicinal Chemistry, and, in particular, in the search for novel compounds with anticancer activity. Similarly, certain tellurium-containing compounds have also exhibited substantial biological activities. Here we provide an overview of the biological activities of seleno- and tellurocompounds including chemopreventive activity, antioxidant or pro-oxidant activity, modulation of the inflammatory processes, induction of apoptosis, modulation of autophagy, inhibition of multidrug efflux pumps such as P-gp, inhibition of cancer metastasis, selective targeting of tumors and enhancement of the cytotoxic activity of chemotherapeutic drugs, as well as overcoming tumor drug resistance. A review of the chemistry of the most relevant seleno- or tellurocompounds with activity against resistant cancers is also presented, paying attention to the synthesis of these compounds and to the preparation of bioactive selenium or tellurium nanoparticles. Based on these data, the use of these seleno- and tellurocompounds is a promising approach in the development of strategies that can drive forward the search for novel therapies or adjuvants of current therapies against drug-resistant cancers.
- Klíčová slova
- Apoptosis, Autophagy, Cancer, Drug combination, Drug development, Efflux pumps, Free radicals, Inflammation, Multidrug resistance (MDR), Selenium, Tellurium,
- MeSH
- chemorezistence MeSH
- lidé MeSH
- mnohočetná léková rezistence MeSH
- nádory * farmakoterapie MeSH
- nanočástice * MeSH
- protinádorové látky * chemie farmakologie terapeutické užití MeSH
- reaktivní formy kyslíku MeSH
- selen * chemie farmakologie terapeutické užití MeSH
- telur chemie farmakologie terapeutické užití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Názvy látek
- protinádorové látky * MeSH
- reaktivní formy kyslíku MeSH
- selen * MeSH
- telur MeSH
Intensive investigation for novel antiproliferative and cytotoxic effective chemical compounds is currently concentrated on structurally modified agents of natural or synthetic source. The selenium derivative of triorganotin compound, triphenyltin isoselenocyanate (TPT-NCSe) caused higher cytotoxicity in hormone sensitive MCF 7 (IC 50-250 nM) in comparison with triple-negative MDA-MB-231 breast carcinoma cell line (IC 50-450 nM) as determined by MTT assay. Measurement of DNA damage showed presence of crosslinks in both cell lines treated by increasing TPT-NCSe concentrations. This compound decreased mitochondrial membrane potential shown by JC-1 staining in a concentration-dependent manner in both cell lines. Activation of caspases-3/7 was observed in MDA-MB-231 cells and was significant only by concentrations causing significant level of crosslinks. On the other hand, migration assay revealed inhibitory effect of viability keeping 100 nM concentration of TPT-NCSe on migration of MDA-MB-231 cells. Our data has shown that this selenium containing triorganotin molecule exerts DNA damage-linked antineoplastic activity in breast carcinoma cell lines studied.
- Klíčová slova
- Apoptosis, Breast cancer, Cytotoxicity, DNA crosslinks, Migration, Triorganotin isoselenocyanates,
- MeSH
- apoptóza MeSH
- lidé MeSH
- MFC-7 buňky MeSH
- nádorové buněčné linie MeSH
- nádory prsu * farmakoterapie MeSH
- organocínové sloučeniny MeSH
- organoselenové sloučeniny MeSH
- proliferace buněk MeSH
- protinádorové látky * chemie farmakologie MeSH
- selen * farmakologie MeSH
- triple-negativní karcinom prsu * metabolismus MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- isoselenocyanate MeSH Prohlížeč
- organocínové sloučeniny MeSH
- organoselenové sloučeniny MeSH
- protinádorové látky * MeSH
- selen * MeSH
- triphenyltin MeSH Prohlížeč
A study was conducted to determine the effect of long-term supplementation with selenium and copper, administered at twice the level used in the standard diet of rats, on the content of selected elements in the femoral bones of healthy rats and rats with implanted LNCaP cancer cells. After an adaptation period, the animals were randomly divided into two experimental groups. The rats in the experimental group were implanted with prostate cancer cells. The rats in the control group were kept in the same conditions as those in the experimental group and fed the same diet, but without implanted cancer cells. The cancer cells (LNCaP) were intraperitoneally implanted in the amount of 1 × 106 (in PBS 0.4 mL) at the age of 90 days. The content of elements in the samples was determined by a quadrupole mass spectrometer with inductively coupled plasma ionization (ICP-MS). In the femoral bones of rats with implanted LNCaP cells, in the case of the standard diet and the copper-enriched diet, there was a marked decreasing trend in the content of the analysed elements relative to the control rats. This may indicate slow osteolysis taking place in the bone tissue. Contrasting results were obtained for the diet enriched with selenium; there was no significant reduction in the level of these elements, and there was even an increase in the concentrations of Fe and K in the bones of rats with implanted LNCaP cells. Particularly, numerous changes in the mineral composition of the bones were generated by enriching the diet with copper. The elements that most often underwent changes (losses) in the bones were cobalt, iron, manganese and molybdenum. The changes observed, most likely induced by the implantation of LNCaP cells, may indicate a disturbance of mineral homeostasis.
- Klíčová slova
- cancer, copper, elements, selenium, supplementation,
- MeSH
- femur MeSH
- krysa rodu Rattus MeSH
- mangan MeSH
- měď analýza farmakologie MeSH
- potravní doplňky MeSH
- selen * farmakologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- mangan MeSH
- měď MeSH
- selen * MeSH
The aim of this study was to compare the effect of parenteral and oral supplementation of Selenium (Se) and vitamin E (VTE) on selected antioxidant parameters in blood and colostrum as well as their effect on the incidence of mastitis in dairy cows during the final phase of gravidity (6 weeks) and first two weeks after calving. For the practical part of the study 36 dairy cows of Slovak pied breed in the second to fourth lactation-gestation cycle were selected. The animals weredivided into three groups: the control (C) and two experimental groups (D1 and D2). The selected groups were treated as follows: in group D1 products containing Se (Selevit inj.) and vitamin E (Erevit sol. inj.) were administered intramuscularly twice, six and three weeks prior to parturition; in group D2 a vitamin-minerals supplement in the form of sodium selenite (Na2SeO3) and dl-α-tocopherol acetate were supplemented orally for six weeks calving. The blood samples were collected from the vena jugularis in dairy cows approximately 42 days before calving (control sampling), on parturition day, and the 14th day after calving. Higher concentrations of Se and VTE were found in the blood plasma samples of both experimental groups collected on the day of parturition. In addition, the orally supplemented group (D2) showed higher Se and α-tocopherol concentrations in blood plasma on the14th day after calving as well a reduction of occurrence of mastitis by about 25 % compared to the control group. The relationship between inflammatory response and oxidative stress was also confirmed. The concentrations of milk malondialdehyde indicating lipid peroxidation during mastitis were significantly higher in milk samples from infected cows than in milk samples from healthy animals in each monitored group. In order to prevent oxidative stress and moderate inflammatory response in dairy cows it is very important to optimally balance their nutritive needs with an appropriate ratio of Se and VTE supplements. Therefore we still recommend supplementation of the cows' postpartum dietwith 0.5 mg of Se/kg dry matter (DM) and 102 mg of dl-α-tocopherol acetate/kg DM to stabilize their optimal blood levels, stimulate the activity of glutathione peroxidase and reduce the incidence of mastitis.
- Klíčová slova
- calving, colostrum, dairy cows, glutathione peroxidase, mastitis, milk malondialdehyde,
- MeSH
- alfa-tokoferol farmakologie MeSH
- antioxidancia farmakologie MeSH
- laktace MeSH
- mastitida skotu * MeSH
- mléčné žlázy zvířat MeSH
- mléko chemie MeSH
- nemoci skotu * MeSH
- potravní doplňky MeSH
- selen * farmakologie MeSH
- skot MeSH
- vitamin E * farmakologie MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- alfa-tokoferol MeSH
- antioxidancia MeSH
- selen * MeSH
- vitamin E * MeSH
Selenium (Se) is a beneficial element, but only when present within its permissible range. Its hyper-accumulation in edible plant parts can cause Se toxicity. This study aimed to develop an agronomic plan for biofortification of rice with Se and reclamation of cadmium (Cd)-contaminated soil, utilizing sodium selenite (Na2SeO3) and cadmium chloride (CdCl2) as soil treatments. Biofortification was performed on two target rice varieties: genotypes 5097A/R2035 and GangYou725, in field trials by applying Cd at a concentration of 0-8 mg kg soil-1 and Se at 0-1 mg kg soil-1. Since these rice varieties have different metabolic specificity, the degree of elemental accumulation, deviations in chlorophyll concentration, activity of photosynthetic apparatus and grain yield were assessed. It was found that application of 1 mg kg-1 Se2O3 decrease Cd content and increased chlorophyll content and photosynthetic activity while grain yield was unaffected by application of the metallic trace-elements. Comparing effects at different stages, we found that the 50% heading stage was most sensitive to metal application. In sum, Se mitigates Cd toxicity, but hyperaccumulation of Se (4 mg kg-1) in polished rice was observed with Cd at 4 and 8 mg kg-1. The elevated level of Cd stress in pot experiments resulted in over-accumulation of Se in the germ and endosperm that poses serious health concerns.
- Klíčová slova
- Accumulation, Cadmium, Health-risk assessment, Morpho-physiological traits, Rice, Selenium,
- MeSH
- kadmium analýza toxicita MeSH
- látky znečišťující půdu * analýza toxicita MeSH
- půda MeSH
- rýže (rod) * MeSH
- selen * farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kadmium MeSH
- látky znečišťující půdu * MeSH
- půda MeSH
- selen * MeSH
In a biological system, nanoparticles (NPs) may interact with biomolecules. Specifically, the adsorption of proteins on the nanoparticle surface may influence both the nanoparticles' and proteins' overall bio-reactivity. Nevertheless, our knowledge of the biocompatibility and risk of exposure to nanomaterials is limited. Here, in vitro and ex ovo biocompatibility of naturally based crosslinked freeze-dried 3D porous collagen/chitosan scaffolds, modified with thermostable fibroblast growth factor 2 (FGF2-STAB®), to enhance healing and selenium nanoparticles (SeNPs) to provide antibacterial activity, were evaluated. Biocompatibility and cytotoxicity were tested in vitro using normal human dermal fibroblasts (NHDF) with scaffolds and SeNPs and FGF2-STAB® solutions. Metabolic activity assays indicated an antagonistic effect of SeNPs and FGF2-STAB® at high concentrations of SeNPs. The half-maximal inhibitory concentration (IC50) of SeNPs for NHDF was 18.9 µg/ml and IC80 was 5.6 µg/ml. The angiogenic properties of the scaffolds were monitored ex ovo using a chick chorioallantoic membrane (CAM) assay and the cytotoxicity of SeNPs over IC80 value was confirmed. Furthermore, the positive effect of FGF2-STAB® at very low concentrations (0.01 µg/ml) on NHDF metabolic activity was observed. Based on detailed in vitro testing, the optimal concentrations of additives in the scaffolds were determined, specifically 1 µg/ml of FGF2-STAB® and 1 µg/ml of SeNPs. The scaffolds were further subjected to antimicrobial tests, where an increase in selenium concentration in the collagen/chitosan scaffolds increased the antibacterial activity. This work highlights the antimicrobial ability and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB® and SeNPs. Moreover, we suggest that these sponges could be used as scaffolds for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration. Due to their antimicrobial properties, these scaffolds are also highly promising for tissue replacement requiring the prevention of infection.
- Klíčová slova
- 3D porous scaffold, Chitosan, Collagen, Fibroblast growth factor 2, Selenium nanoparticles,
- MeSH
- antibakteriální látky MeSH
- biokompatibilní materiály farmakologie MeSH
- buněčné linie MeSH
- chitosan farmakologie MeSH
- fibroblastový růstový faktor 2 farmakologie MeSH
- fibroblasty účinky léků MeSH
- hojení ran MeSH
- kolagen farmakologie MeSH
- lidé MeSH
- nanočástice chemie terapeutické užití MeSH
- poréznost MeSH
- selen chemie farmakologie MeSH
- testování materiálů MeSH
- tkáňové inženýrství metody MeSH
- tkáňové podpůrné struktury * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- biokompatibilní materiály MeSH
- chitosan MeSH
- fibroblastový růstový faktor 2 MeSH
- kolagen MeSH
- selen MeSH
This study focused on the idea that the toxic effect of zearalenone (ZEA) and the protective actions of the brassinosteroid - 24-epibrassinolide (EBR) as well as selenium are dependent on its accumulation in chloroplasts to a high degree. These organelles were isolated from the leaves of oxidative stress-sensitive and stress-tolerant wheat cultivars that had been grown from grains that had been incubated in a solution of ZEA (30 μM), Na2SeO4 (Se, 10 μM), EBR (0.1 μM) or in a mixture of ZEA with Se or EBR. Ultra-high performance liquid chromatography techniques indicated that ZEA was adsorbed in higher amounts in the chloroplasts in the sensitive rather than tolerant cultivar. Although the brassinosteroids and Se were also accumulated in the chloroplasts, higher levels were only found in the tolerant cultivar. The application of EBR increased the homocastasterone content, especially in the chloroplasts of the tolerant plant and after the addition of ZEA. The presence of both protectants caused a decrease in the ZEA content in studied organelles and resulted in diminishing of the oxidative stress (i.e. changes in the activity of the antioxidative enzymes). Moreover, a recovery of photosystem II and decrease in the negative impact of ZEN on Hsp90 transcript accumulation was observed in plants.
- Klíčová slova
- Brassinosteroids, Chloroplasts, Hsp90, Photosynthesis, Selenium, Wheat, Zearalenone,
- MeSH
- antioxidancia metabolismus MeSH
- brassinosteroidy metabolismus farmakokinetika farmakologie MeSH
- chlorofyl a metabolismus MeSH
- chloroplasty účinky léků MeSH
- enzymy metabolismus MeSH
- karotenoidy metabolismus MeSH
- oxidační stres účinky léků MeSH
- proteiny tepelného šoku HSP90 genetika MeSH
- pšenice účinky léků metabolismus MeSH
- rostlinné proteiny genetika MeSH
- selen farmakokinetika farmakologie MeSH
- steroidy heterocyklické farmakokinetika farmakologie MeSH
- zearalenon farmakokinetika toxicita MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antioxidancia MeSH
- brassinolide MeSH Prohlížeč
- brassinosteroidy MeSH
- chlorofyl a MeSH
- enzymy MeSH
- karotenoidy MeSH
- proteiny tepelného šoku HSP90 MeSH
- rostlinné proteiny MeSH
- selen MeSH
- steroidy heterocyklické MeSH
- zearalenon MeSH
