We compared ganglioside profiles of animal tumours (B16 and Cloudman S91 murine melanomas, Bomirski Syrian hamster melanoma), which are widely used as models of human melanoma, and of their melanosomal fractions. A ganglioside fraction was extracted and purified and the amount of each component ganglioside was assessed by thin-layer chromatography. GM3 was the dominant ganglioside species in the murine melanomas studied. Unlike human melanomas, the GD3 expression in mouse melanomas was low. GD3 and GM3 were major gangliosides in Bomirski hamster melanoma. Alkali-labile O-acetyl-GD3, a melanoma-specific ganglioside, was detected only in Bomirski melanoma. GD2, which in human melanoma is seen as a distinct signal of tumour progression, was not found in the animal melanomas studied. Melanosomes isolated from B16 and Bomirski melanomas contained GM3 and GD3 as their major ganglioside components. These data extend the group of common antigenic determinants shared by melanosomes and cell surface of pigment cells.

• MeSH
• antigeny nádorové imunologie   MeSH
• G(M3) gangliosid analýza   MeSH
• gangliosidy analýza   MeSH
• křečci praví MeSH
• křeček rodu Mesocricetus MeSH
• melanocyty chemie   MeSH
• melanom experimentální chemie   MeSH
• myši inbrední C57BL MeSH
• myši inbrední DBA MeSH
• myši MeSH
• nádorové biomarkery MeSH
• nádory kůže chemie   MeSH
• zvířata MeSH
• Check Tag
• křečci praví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• antigeny nádorové MeSH
• G(M3) gangliosid MeSH
• ganglioside, GD3 MeSH Prohlížeč
• gangliosidy MeSH
• nádorové biomarkery MeSH