Screening for metabolic abnormalities of Trp has been introduced using SPE pre-treatment, TLC and/or two HPLC procedures. The excretory pattern in urine (occasionally also plasma and CSF levels) has been followed in a group of 390 children showing various symptoms of a metabolic defect and in 195 patients with skin diseases, namely those associated with photosensitivity, such as porphyria, vitiligo, alopecia, psoriasis, erythematodes, and others. Excretory abnormalities of either indican, kynurenine, 3-hydroxyanthranilic acid or indolylacryloylglycine have been occasionally combined with myopathy, seizures, liver and intestinal symptoms. Several indoles and kynurenine derivatives present changes in the alopecia group and the vitiliginous patients.

• MeSH
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• kožní nemoci metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• poruchy fotosenzitivity metabolismus   MeSH
• předškolní dítě MeSH
• senioři MeSH
• studie případů a kontrol MeSH
• tryptofan analogy a deriváty   analýza   metabolismus   MeSH
• vrozené poruchy metabolismu metabolismus   MeSH
• vysokoúčinná kapalinová chromatografie metody   MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• předškolní dítě MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• tryptofan MeSH

Many compounds related to L-tryptophan (L-TRP) have interesting biological or pharmacological activity, and their abnormal neurotransmission seems to be linked to a wide range of neurodegenerative and psychiatric diseases. A high-throughput method based on ultra-high performance liquid chromatography connected to electrospray tandem mass spectrometry (UHPLC-ESI-MS/MS) was developed for the quantitative analysis of L-TRP and 16 of its metabolites in human serum and cerebrospinal fluid (CSF), representing both major and minor routes of L-TRP catabolism. The combination of a fast LC gradient with selective tandem mass spectrometry enabled accurate analysis of almost 100 samples in 24h. The standard isotope dilution method was used for quantitative determination. The method's lower limits of quantification for serum and cerebrospinal fluid ranged from 0.05 to 15nmol/L and 0.3 to 45nmol/L, respectively. Analytical recoveries ranged from 10.4 to 218.1% for serum and 22.1 to 370.0% for CSF. The method's accuracy ranged from 82.4 to 128.5% for serum matrix and 90.7 to 127.7% for CSF matrix. All intra- and inter-day coefficients of variation were below 15%. These results demonstrate that the new method is capable of quantifying endogenous serum and CSF levels of a heterogeneous group of compounds spanning a wide range of concentrations. The method was used to determine the physiological levels of target analytes in serum and CSF samples from 18 individuals, demonstrating its reliability and potential usefulness in large-scale epidemiological studies.

• Klíčová slova
• Cerebrospinal fluid, Neurochemicals, Serum, Tryptophan metabolism, UHPLC–MS/MS,
• MeSH
• biochemická analýza krve metody   MeSH
• chemické techniky analytické metody   MeSH
• indikátorové diluční techniky MeSH
• izotopy MeSH
• lidé MeSH
• reprodukovatelnost výsledků MeSH
• tandemová hmotnostní spektrometrie * MeSH
• tryptofan krev   mozkomíšní mok   MeSH
• vysokoúčinná kapalinová chromatografie * MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• izotopy MeSH
• tryptofan MeSH