The validity of the principle of homeoviscous adaptation for Bacillus subtilis was tested by comparing fluorescence anisotropy (1,6-diphenyl-1,3,5-hexatriene) and electron-spin resonance (16-doxylstearate) measurements carried out in isolated plasma membranes and in phospholipid fractions. The physical measurements were supplemented by fatty-acid analysis. The results support our previous findings on intact cells. The thermoadaptive mechanism of B. subtilis manifested as an increase in relative proportion of branched anteiso-C15 and anteiso-C17 fatty acids, are not strong enough to compensate for the marked physical change of membrane fluidity induced by temperature decrease.

• MeSH
• Bacillus subtilis analýza   fyziologie   MeSH
• elektronová paramagnetická rezonance MeSH
• fluidita membrány * MeSH
• fluorescenční polarizace MeSH
• fosfolipidy analýza   MeSH
• fyziologická adaptace * MeSH
• mastné kyseliny analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• fosfolipidy MeSH
• mastné kyseliny MeSH