The validity of the principle of homeoviscous adaptation for Bacillus subtilis was tested by comparing fluorescence anisotropy (1,6-diphenyl-1,3,5-hexatriene) and electron-spin resonance (16-doxylstearate) measurements carried out in isolated plasma membranes and in phospholipid fractions. The physical measurements were supplemented by fatty-acid analysis. The results support our previous findings on intact cells. The thermoadaptive mechanism of B. subtilis manifested as an increase in relative proportion of branched anteiso-C15 and anteiso-C17 fatty acids, are not strong enough to compensate for the marked physical change of membrane fluidity induced by temperature decrease.

Department of Microbiology Faculty of Science Charles University Prague

Zobrazit více v PubMed

Biochim Biophys Acta. 1972 May 23;270(1):32-9 PubMed

Eur J Biochem. 1967 Nov;2(4):448-53 PubMed

J Bacteriol. 1977 Nov;132(2):473-84 PubMed

Biochimie. 1976;58(1-2):99-108 PubMed

J Biol Chem. 1973 Oct 10;248(19):6885-95 PubMed

Proc Natl Acad Sci U S A. 1974 Feb;71(2):522-5 PubMed

Folia Microbiol (Praha). 1988;33(3):161-9 PubMed

Eur J Biochem. 1978 Apr 17;85(2):451-6 PubMed

Folia Microbiol (Praha). 1988;33(1):1-9 PubMed

Methods Enzymol. 1981;72:5-7 PubMed

Long-term adaptation of Bacillus subtilis 168 to extreme pH affects chemical and physical properties of the cellular membrane

Detection of lateral heterogeneity in the cytoplasmic membrane of Bacillus subtilis

Rotational relaxation rate of 1,6-diphenyl-1,3,5-hexatriene in cytoplasmic membranes of Bacillus subtilis. A new model of heterogeneous rotations