A method for determination of membrane fluidity (microviscosity) in Bacillus subtilis cytoplasmic membrane under in vivo conditions is described. The membranes were labelled with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene during the exponential phase of growth. Fluorescence anisotropy measurements were carried out in an intact cell suspension having absorbance A as high as 0.2-0.3 (corresponding to a cell concentration of 100-300/nL).

Department of Microbiology Faculty of Science Charles University Prague

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Biochemistry. 1978 Aug 22;17(17):3581-6 PubMed

J Mol Biol. 1974 Jan 5;85(4):603-15 PubMed

Biochim Biophys Acta. 1982 Jun 28;688(3):764-74 PubMed

Biochim Biophys Acta. 1985 Apr 22;845(1):60-7 PubMed

Biochim Biophys Acta. 1976 Apr 16;433(1):133-49 PubMed

J Bacteriol. 1962 Dec;84(6):1260-7 PubMed

Biochemistry. 1979 Feb 6;18(3):520-7 PubMed

Proc Natl Acad Sci U S A. 1977 Mar;74(3):1072-6 PubMed

Biochim Biophys Acta. 1980 Sep 2;601(1):1-10 PubMed

Biochim Biophys Acta. 1978 Dec 15;515(4):367-94 PubMed

FEBS Lett. 1978 Apr 15;88(2):187-91 PubMed

FEBS Lett. 1983 Jul 25;158(2):229-33 PubMed

Biochem Biophys Res Commun. 1977 Jun 6;76(3):869-74 PubMed

Annu Rev Biophys Bioeng. 1976;5:205-38 PubMed

Proc Natl Acad Sci U S A. 1979 Oct;76(10):5313-6 PubMed

J Biol Chem. 1974 Apr 25;249(8):2652-7 PubMed

Biochim Biophys Acta. 1980 May 23;598(2):237-46 PubMed

Rotational relaxation rate of 1,6-diphenyl-1,3,5-hexatriene in cytoplasmic membranes of Bacillus subtilis. A new model of heterogeneous rotations

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