Mammalian dentition exhibits distinct heterodonty, with more simple teeth located in the anterior area of the jaw and more complex teeth situated posteriorly. While some region-specific differences in signalling have been described previously, here we performed a comprehensive analysis of gene expression at the early stages of odontogenesis to obtain complete knowledge of the signalling pathways involved in early jaw patterning. Gene expression was analysed separately on anterior and posterior areas of the lower jaw at two early stages (E11.5 and E12.5) of odontogenesis. Gene expression profiling revealed distinct region-specific expression patterns in mouse mandibles, including several known BMP and FGF signalling members and we also identified several new molecules exhibiting significant differences in expression along the anterior-posterior axis, which potentially can play the role during incisor and molar specification. Next, we followed one of the anterior molecules, SATB2, which was expressed not only in the anterior mesenchyme where incisor germs are initiated, however, we uncovered a distinct SATB2-positive region in the mesenchyme closely surrounding molars. Satb2-deficient animals demonstrated defective incisor development confirming a crucial role of SATB2 in formation of anterior teeth. On the other hand, ectopic tooth germs were observed in the molar area indicating differential effect of Satb2-deficiency in individual jaw regions. In conclusion, our data provide a rich source of fundamental information, which can be used to determine molecular regulation driving early embryonic jaw patterning and serve for a deeper understanding of molecular signalling directed towards incisor and molar development.

• Klíčová slova
• Incisor, Lower jaw, Microarray, Molar, Mouse, Satb2,
• MeSH
• mandibula * metabolismus   embryologie   MeSH
• myši MeSH
• odontogeneze * genetika   MeSH
• řezáky metabolismus   embryologie   růst a vývoj   MeSH
• rozvržení tělního plánu genetika   MeSH
• signální transdukce MeSH
• stanovení celkové genové exprese * MeSH
• transkripční faktory * genetika   metabolismus   MeSH
• vazebné proteiny DNA v oblastech připojení k matrix * genetika   metabolismus   MeSH
• vývojová regulace genové exprese * MeSH
• zuby metabolismus   embryologie   růst a vývoj   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• SATB2 protein, mouse MeSH Prohlížeč
• transkripční faktory * MeSH
• vazebné proteiny DNA v oblastech připojení k matrix * MeSH