Polyurethene (PU) catheters were coated with 3-(trimethoxysilyl)-propyldimethyloctadecylammonium chloride (QAS) by means of a multistep process which involved a vapor phase plasma-induced graft-polymerization of acrylic acid (AAc). The AAc coating, whose stability in aqueous media was assessed by immersion in Phospate Buffer Saline (PBS), was characterized by means of Attenuated Total Reflectance Fourier Transform Infrared (ATR/FTIR) spectroscopy. Moreover, the COOH surface density was evaluated by a colorimetric assay with Methylene Blue. Carrying a negative charge at neutral pH, AAc coatings were proficient in positively charged molecules (like QAS) adsorption. ATR/FTIR spectroscopy and a colorimetric assay with Bromophenol Blue allowed us to verify the presence and the uniformity of the QAS coating on the PU catheters and the positive effect of the AAc graft-polymerization on the QAS adsorption. Morphological characterization of the QAS-modified catheters was performed by means of Atomic Force Microscopy (AFM). QAS-coated catheters displayed in vitro antimicrobial activity against Gram-negative Escherichia coli bacterial cells.

• Klíčová slova
• AFM, ATR/FTIR, Acrylic acid, Escherichia coli, Plasma-induced graft-polymerization, Quaternary ammonium silane,
• MeSH
• akryláty chemie   farmakologie   MeSH
• antibakteriální látky chemie   farmakologie   MeSH
• biokompatibilní potahované materiály chemie   farmakologie   MeSH
• Escherichia coli účinky léků   MeSH
• infekce vyvolané Escherichia coli prevence a kontrola   MeSH
• katétry mikrobiologie   MeSH
• kvartérní amoniové sloučeniny chemie   farmakologie   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• polyurethany chemie   farmakologie   MeSH
• povrchové vlastnosti MeSH
• silany chemie   farmakologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• acrylic acid MeSH Prohlížeč
• akryláty MeSH
• antibakteriální látky MeSH
• biokompatibilní potahované materiály MeSH
• kvartérní amoniové sloučeniny MeSH
• polyurethany MeSH
• silany MeSH

Currently, one of the most promising treatments of lipopolysaccharides (LPS)-induced sepsis is based on hemofiltration. Nevertheless, proteins rapidly adsorbed on the artificial surface of membranes which leads to activation of coagulation impairing effective scavenging of the endotoxins. To overcome this challenge, we designed polymer-brush-coated microparticles displaying antifouling properties and functionalized them with polymyxin B (PMB) to specifically scavenge LPS the most common endotoxin. Poly[( N-(2-hydroxypropyl) methacrylamide)- co-(carboxybetaine methacrylamide)] brushes were grafted from poly(glycidyl methacrylate) microparticles using photoinduced single-electron transfer living radical polymerization (SET-LRP). Notably, only parts-per-million of copper catalyst were necessary to achieve brushes able to repel adsorption of proteins from blood plasma. The open porosity of the particles, accessible to polymerization, enabled us to immobilize sufficient PMB to selectively scavenge LPS from blood plasma.

• MeSH
• adsorpce MeSH
• akrylamidy metabolismus   MeSH
• biokompatibilní potahované materiály farmakologie   MeSH
• bioznečištění prevence a kontrola   MeSH
• epoxidové sloučeniny metabolismus   MeSH
• krevní plazma metabolismus   MeSH
• lidé MeSH
• lipopolysacharidy metabolismus   MeSH
• methakryláty metabolismus   MeSH
• polymerizace účinky léků   MeSH
• polymery chemie   MeSH
• polymyxin B farmakologie   MeSH
• povrchové vlastnosti účinky léků   MeSH
• proteiny metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• akrylamidy MeSH
• biokompatibilní potahované materiály MeSH
• epoxidové sloučeniny MeSH
• glycidyl methacrylate MeSH Prohlížeč
• lipopolysacharidy MeSH
• methacrylamide MeSH Prohlížeč
• methakryláty MeSH
• polymery MeSH
• polymyxin B MeSH
• proteiny MeSH