BACKGROUND: Extensive wound coverage still represents a challenge for contemporary medicine. We demonstrate the results of a clinical trial of the grafting of cultured keratinocytes directly on a polymer cultivation support in the treatment of skin defects in seriously burned patients and in patients with trophic ulcers. METHODS: Wound closure was evaluated clinically. The morphology and phenotypic pattern of the reconstructed epidermis, including the basal lamina, as well as the presence of Langerhans cells, were evaluated immunocytochemically using a panel of monoclonal antibodies. RESULTS: All layers of the reconstructed epidermis were normally differentiated (cytokeratin immunocytochemistry). The basal lamina contained collagen type IV and laminin. The reconstructed epidermis was extensively colonized by Langerhans cells. CONCLUSIONS: The results of the described technology are encouraging, especially in patients after a burn injury. The described procedure is suitable for the treatment of skin defects in clinical practice.

• MeSH
• dítě MeSH
• dospělí MeSH
• epidermis chirurgie   MeSH
• hojení ran MeSH
• keratinocyty transplantace   MeSH
• kojenec MeSH
• kožní vředy chirurgie   MeSH
• kultivované buňky MeSH
• lidé středního věku MeSH
• lidé MeSH
• odběr tkání a orgánů rehabilitace   MeSH
• polymery MeSH
• popálení chirurgie   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• tkáňové inženýrství * MeSH
• transplantace buněk * MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• polymery MeSH