Reconstruction of epidermis by grafting of keratinocytes cultured on polymer support--clinical study
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12653921
DOI
10.1046/j.1365-4362.2003.01792.x
PII: 1792
Knihovny.cz E-resources
- MeSH
- Child MeSH
- Adult MeSH
- Epidermis surgery MeSH
- Wound Healing MeSH
- Keratinocytes transplantation MeSH
- Infant MeSH
- Skin Ulcer surgery MeSH
- Cells, Cultured MeSH
- Middle Aged MeSH
- Humans MeSH
- Tissue and Organ Harvesting rehabilitation MeSH
- Polymers MeSH
- Burns surgery MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Tissue Engineering * MeSH
- Cell Transplantation * MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Polymers MeSH
BACKGROUND: Extensive wound coverage still represents a challenge for contemporary medicine. We demonstrate the results of a clinical trial of the grafting of cultured keratinocytes directly on a polymer cultivation support in the treatment of skin defects in seriously burned patients and in patients with trophic ulcers. METHODS: Wound closure was evaluated clinically. The morphology and phenotypic pattern of the reconstructed epidermis, including the basal lamina, as well as the presence of Langerhans cells, were evaluated immunocytochemically using a panel of monoclonal antibodies. RESULTS: All layers of the reconstructed epidermis were normally differentiated (cytokeratin immunocytochemistry). The basal lamina contained collagen type IV and laminin. The reconstructed epidermis was extensively colonized by Langerhans cells. CONCLUSIONS: The results of the described technology are encouraging, especially in patients after a burn injury. The described procedure is suitable for the treatment of skin defects in clinical practice.
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