The p53 protein can control cell cycle progression, programmed cell death, and differentiation of many cell types. Ectopic expression of p53 can resume capability of cell cycle arrest, differentiation, and apoptosis in various leukemic cell lines. In this work, we expressed human p53 protein in v-Myb-transformed chicken monoblasts. We found that even this protein possessing only 53% amino acid homology to its avian counterpart can significantly alter morphology and physiology of these cells causing the G2-phase cell cycle arrest and early monocytic differentiation. Our results document that the species-specific differences of the p53 molecules, promoters/enhancers, and co-factors in avian and human cells do not interfere with differentiation- and cell cycle arrest promoting capabilites of the p53 tumor suppressor even in the presence of functional v-Myb oncoprotein. The p53-induced differentiation and cell cycle arrest of v-Myb-transformed monoblasts are not associated with apoptosis suggesting that the p53-driven pathways controlling apoptosis and differentiation/proliferation are independent.

• MeSH
• apoptóza genetika   MeSH
• buněčná diferenciace genetika   fyziologie   MeSH
• buněčný cyklus genetika   MeSH
• G2 fáze genetika   MeSH
• inhibitory růstu genetika   fyziologie   MeSH
• kur domácí MeSH
• lidé MeSH
• monocyty cytologie   MeSH
• nádorový supresorový protein p53 genetika   fyziologie   MeSH
• onkogenní proteiny v-myb genetika   MeSH
• proliferace buněk * MeSH
• signální transdukce genetika   MeSH
• transfekce MeSH
• transformované buněčné linie MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• inhibitory růstu MeSH
• nádorový supresorový protein p53 MeSH
• onkogenní proteiny v-myb MeSH