Clonal haematopoiesis of indeterminate potential (CHIP) may predispose for the development of therapy-related myeloid neoplasms (t-MN). Using target next-generation sequencing (t-NGS) panels and digital droplet polymerase chain reactions (ddPCR), we studied the myeloid gene mutation profiles of patients with chronic lymphocytic leukaemia (CLL) who developed a t-MN after treatment with chemo-(immuno)therapy. Using NGS, we detected a total of 30 pathogenic/likely pathogenic (P/LP) variants in 10 of 13 patients with a t-MN (77%, median number of variants for patient: 2, range 0-6). The prevalence of CHIP was then backtracked in paired samples taken at CLL diagnosis in eight of these patients. Six of them carried at least one CHIP-variant at the time of t-MN (median: 2, range: 1-5), and the same variants were present in the CLL sample in five cases. CHIP variants were present in 34 of 285 patients from a population-based CLL cohort, which translates into a significantly higher prevalence of CHIP in patients with a CLL who developed a t-MN, compared to the population-based cohort (5/8, 62.5% vs. 34/285, 12%, p = 0.0001). Our data show that CHIP may be considered as a novel parameter affecting treatment algorithms in patients with CLL, and highlight the potential of using chemo-free therapies in CHIP-positive cases.

• Klíčová slova
• CHIP and FCR, CLL, t-MN,
• MeSH
• chronická lymfatická leukemie * farmakoterapie   genetika   patologie   MeSH
• klonální hematopoéza genetika   MeSH
• lidé MeSH
• mutace MeSH
• protokoly antitumorózní kombinované chemoterapie škodlivé účinky   MeSH
• rizikové faktory MeSH
• sekundární malignity * etiologie   genetika   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• Klíčová slova
• CATALASE/chemistry *, LIVER/chemistry *,
• MeSH
• játra chemie   MeSH
• katalasa chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa MeSH

Three recently isolated catalase-negative mutants of Hansenula polymorpha lost the ability to grow on methanol but grew in media containing glucose, ethanol or glycerol. Their incubation in a medium with methanol resulted in an accumulation of hydrogen peroxide and cell death. During growth of a catalase-negative mutant in chemostat on a mixture of methanol and glucose, neither H2O2 accumulation nor cell death were observed up to the molar ratio of 10:1 of the two substrates. Cytochrome-c peroxidase and NADH-peroxidase activities were detected in the cells. In methylotrophic yeasts, catalase seems to be an enzyme characteristic of the metabolism of methanol but not needed for the metabolism of multicarbon substrates. The hydrogen peroxide produced during growth of the mutants on mixed substrates is detoxified by cytochrome-c peroxidase and other peroxidases.

• MeSH
• katalasa genetika   MeSH
• mutace * MeSH
• Pichia enzymologie   genetika   růst a vývoj   MeSH
• subcelulární frakce enzymologie   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa MeSH

The effects of altered thyroid state on the antioxidant defense system in the liver of differently aged rats were examined. Male rats aged 15, 45 and 75 days were treated with L-thyroxine, T(4) (40 microg/100 g body mass, s.c., one dose per day) for 14 days (finally aged 30, 60 and 90 days, respectively). The following antioxidant defense enzymes were measured: superoxide dismutases (both copper zinc, CuZn-SOD and manganese containing, Mn-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), glutathione reductase (GR), as well as the content of low molecular mass antioxidant glutathione (GSH). The effect of T(4) on antioxidant defense system in the liver differs with respect to age. T(4) treatment decreased CAT and GST activities, as well as the content of GSH in animals aged 60 and 90 days. The same treatment elevated GR activity in rats at 30 days of age, this phenomenon was not observed in older animals. The different response of immature rats to thyroxine compared to older animals could be attributed to the differences in thyroxine metabolism and the developmental pattern. Direct effect of T(4) on mature rats can be considered as a part of its overall catabolic action.

• MeSH
• antioxidancia metabolismus   MeSH
• glutathion metabolismus   MeSH
• glutathionperoxidasa metabolismus   MeSH
• glutathionreduktasa metabolismus   MeSH
• glutathiontransferasa metabolismus   MeSH
• inbrední kmeny potkanů MeSH
• játra účinky léků   enzymologie   metabolismus   MeSH
• katalasa metabolismus   MeSH
• krysa rodu Rattus MeSH
• superoxiddismutasa metabolismus   MeSH
• thyroxin farmakologie   MeSH
• věkové faktory MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• glutathion MeSH
• glutathionperoxidasa MeSH
• glutathionreduktasa MeSH
• glutathiontransferasa MeSH
• katalasa MeSH
• superoxiddismutasa MeSH
• thyroxin MeSH

• Klíčová slova
• CATALASE/cerebrospinal fluid *,
• MeSH
• katalasa mozkomíšní mok   MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa MeSH

• Klíčová slova
• CATALASE/urine *,
• MeSH
• katalasa moč   MeSH
• lidé MeSH
• tělesné tekutiny * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa MeSH

• Klíčová slova
• CATALASE *, MYCOBACTERIUM TUBERCULOSIS/metabolism *,
• MeSH
• katalasa * MeSH
• kvalitativní výzkum * MeSH
• Mycobacterium tuberculosis metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa * MeSH

Green biomass of young barley plants exhibited statistically significant higher activity of superoxide dismutase (SOD) and catalase (CAT) at sampling I (in the phase of plant development DC 29) compared to the later sampling II (DC 31). Significant effects of varieties, years and interactions of the studied factors on the activity of the studied antioxidants were determined. During the experiment period (2005-2007), the variety Sebastian provided statistically significant higher average SOD activity (486 U.g-1) versus the variety Malz (416 U.g-1 dry matter) and line KM1910 (418 U.g-1 dry matter). No statistically significant difference was recorded between the latter two varieties. Average catalase activity of the varieties did not show any significant difference. Significantly higher CAT activity in the sampling I was recorded on average of years and locations in the variety Sebastian and hull-less line KM1910 (935 and 907 U.g-1) compared to the variety Malz (675 U.g-1). We can state that green biomass of young spring barley plants taken during the growth phase DC 29 was a significant source of enzymes catalase and superoxide dismutase in the course of the experiment (2005-2007).

• MeSH
• antioxidancia metabolismus   MeSH
• ječmen (rod) enzymologie   MeSH
• katalasa metabolismus   MeSH
• semenáček MeSH
• superoxiddismutasa metabolismus   MeSH
• výhonky rostlin enzymologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• katalasa MeSH
• superoxiddismutasa MeSH

• MeSH
• katalasa krev   MeSH
• lidé MeSH
• schizofrenie krev   farmakoterapie   enzymologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa MeSH

• Klíčová slova
• CATALASE *, CEREBROSPINAL FLUID *,
• MeSH
• katalasa * MeSH
• lidé MeSH
• mozkomíšní mok * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• katalasa * MeSH