The author describes work with two apparatuses, Urilux and Miditron, which evaluate by means of reflex photometry as many as ten parameters on diagnostic strips dipped in the examined urine sample. The first apparatus is suitable for doctors' surgeries or in-patient departments, the second larger one for diagnostic laboratories.

• MeSH
• analýza moči přístrojové vybavení   MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH

OBJECTIVES: Stability of concentrations of urinary stone-related metabolites was analyzed from samples of recurrent urinary stone formers to assess necessity and effectiveness of urine acidification during collection and storage. METHODS: First-morning urine was collected from 20 adult calcium-stone forming patients at Tomas Bata Hospital in the Czech Republic. Urine samples were analyzed for calcium, magnesium, inorganic phosphate, uric acid, sodium, potassium, chloride, citrate, oxalate, and urine particles. The single-voided specimens were collected without acidification, after which they were divided into three groups for storage: samples without acidification ("NON"), acidification before storage ("PRE"), or acidification after storage ("POST"). The analyses were conducted on the day of arrival (day 0, "baseline"), or after storage for 2 or 7 days at room temperature. The maximum permissible difference (MPD) was defined as ±20 % from the baseline. RESULTS: The urine concentrations of all stone-related metabolites remained within the 20 % MPD limits in NON and POST samples after 2 days, except for calcium in NON sample of one patient, and oxalate of three patients and citrate of one patient in POST samples. In PRE samples, stability failed in urine samples for oxalate of three patients, and for uric acid of four patients after 2 days. Failures in stability often correlated with high baseline concentrations of those metabolites in urine. CONCLUSIONS: Detailed procedures are needed to collect urine specimens for analysis of urinary stone-related metabolites, considering both patient safety and stability of those metabolites. We recommend specific preservation steps.

• Klíčová slova
• kidney stone formers, preanalytical, specimen preservation, stability, urine, urolithiasis,
• MeSH
• analýza moči metody   MeSH
• dospělí MeSH
• koncentrace vodíkových iontů MeSH
• kyselina močová moč   MeSH
• lidé středního věku MeSH
• lidé MeSH
• močové kameny * moč   MeSH
• odběr biologického vzorku metody   MeSH
• recidiva * MeSH
• sbírání vzorku moči metody   MeSH
• senioři MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• kyselina močová MeSH

This study evaluates the performance of microscopic part of a hybrid analyzer FUS-2000 (Dirui Industrial Co., Changchun, China), its analytical properties and particle recognition. The evaluation of trueness, repeatability, detection limit, carry-over, linearity range and analytical stability was performed according to Dirui protocol guidelines designed by Dirui Company to guarantee the quality of the instrument. Trueness for low, medium and high-value concentrations was calculated with bias of 15.5, 4.7 and -6.6%, respectively. Detection limit of 5 Ery/μl was confirmed. Coefficient of variation of 11.0, 5.2 and 3.8% was measured for within-run repeatability of low, medium and high concentration. Between-run repeatability for daily quality control had coefficient of variation of 3.0%. Carry-over did not exceed 0.05%. Linearity was confirmed for range of 0-16,000 particles/μl (R2 = 0.9997). The analytical stability had coefficient of variation of 4.3%. Out of 1258 analyzed urine samples, 362 positive were subjected to light microscopy urine sediment analysis and compared to the analyzer results. Cohen's kappa coefficients were calculated to express the concordance. Squared kappa coefficient was 0.927 (red blood cells), 0.888 (white blood cells), 0.908 (squamous epithelia), 0.634 (transitional epithelia), 0.628 (hyaline casts), 0.843 (granular casts) and 0.623 (bacteria). Single kappa coefficients were 0.885 (yeasts) and 0.756 (crystals), respectively. Aforementioned results show good analytical performance of the analyzer and tight agreement with light microscopy of urine sediment.

• Klíčová slova
• Laboratory routines and automation, analytical performance, laboratory statistics, medical biochemistry, microscopy, urinalysis,
• MeSH
• analýza moči přístrojové vybavení   MeSH
• lidé MeSH
• referenční hodnoty MeSH
• směrnice jako téma MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH

• Klíčová slova
• URINE *,
• MeSH
• analýza moči * MeSH
• lidé MeSH
• moč * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Quantitative bacterial culture (QBC) is the gold standard for determining urinary tract infections. However, the majority of urine samples were sent to a lab for further bacterial culture. Therefore, the storage condition was vital to maintain the quality and accuracy of the urine samples. The main objective of our study was to examine the urine QBC outcomes of (1) immediate culture, (2) culture after urine sample storage at ambient temperature for 24 h, and (3) culture after urine sample storage at 4 °C for 24 hours. There were 49 feline samples and 30 canine samples included in this study. All QBC samples kept at ambient temperature and refrigerator were consistent with immediate QBC in cats. Eight positive results from immediate QBC were in accordance with refrigerated results in dogs. There were ten positive results in the room-temperature sample with two false-positive results. Our study showed that storing conditions at room temperature or refrigeration for 24 h does not impact the results of QBC in cat urine samples. For dog samples, chilled samples have a higher accuracy rate than room temperature samples, although the overall agreement was still satisfactory.

• Klíčová slova
• canine, feline, urinalysis, urinary tract infections,
• Publikační typ
• časopisecké články MeSH

• Klíčová slova
• LUNGS/anatomy and histology *,
• MeSH
• analýza moči * MeSH
• histologické techniky * MeSH
• lidé MeSH
• plíce anatomie a histologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

The breed can influence the results of haematological and biochemical blood tests, with sighthounds traditionally mentioned. It may also affect certain urinary parameters. This study aimed to compare urinary protein and creatinine concentrations and their ratio (UPC, urine protein to creatinine ratio) between sighthounds and non-sighthounds and to evaluate these parameters in various sighthound breeds. Urine samples from clinically healthy dogs were collected via normal voiding, representing both sighthound and non-sighthound breeds. The protein and creatinine concentrations in the urine samples were determined, and their ratio was subsequently calculated. A total of 191 urine samples from sighthounds and 90 urine samples from non-sighthound breeds used as a control group were evaluated in the study. In sighthounds, significantly lower urinary protein concentration (248.8 mg/l and 299.8 mg/l, respectively; P = 0.045) and significantly higher urinary creatinine concentration (23.0 mmol/l and 17.5 mmol/l, respectively; P = 0.000) and lower UPC values (0.13 and 0.18, respectively; P = 0.000) were observed in comparison to the entire control group. The UPC values were found to be significantly lower in Greyhounds and Spanish Greyhounds compared with non-sighthounds. Although statistically significant changes were identified, they are unlikely to be of great clinical importance.

• Klíčová slova
• Greyhound, Spanish Greyhound, Whippet, proteinuria, urinalysis,
• Publikační typ
• časopisecké články MeSH

• Klíčová slova
• BACTERIOLOGICAL TECHNICS *, URINARY TRACT INFECTIONS *,
• MeSH
• analýza moči * MeSH
• bakteriologické techniky * MeSH
• infekce močového ústrojí * MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

• Klíčová slova
• LIVER CIRRHOSIS/urine *, POTASSIUM/urine *, SODIUM/urine *,
• MeSH
• analýza moči * MeSH
• draslík moč   MeSH
• jaterní cirhóza moč   MeSH
• lidé MeSH
• sodík moč   MeSH
• tělesné tekutiny * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• draslík MeSH
• sodík MeSH

Urinary N-acetyl-β-D-glucosaminidase (NAG) activity is associated with kidney disease. In our study, we focused on evaluating the ratio of NAG to the urinary creatinine concentration (NAG/Cr) in the German Shepherd breed. Sixty-two healthy dogs and thirteen dogs with chronic kidney disease (CKD) were examined. The healthy dogs were divided into groups based on their sex and age (puppies and adolescent dogs under 2 years, adult dogs from 2 to 6 years and seniors over 6 years), while the dogs with CKD were divided based on the disease stage (CKD stage 1 and 2). No significant difference was detected between the males and females. Regarding the age dependence, significantly higher values were found in dogs older than 6 years (P = 0.020 3) compared to dogs aged 2-6 years. When comparing the healthy dogs to the dogs with CKD, the lowest NAG/Cr ratio was observed in the dogs with CKD stage 2. The reference interval for NAG/Cr in the German Shepherd was established in the range of 0.78-7.86 U/gCr. We observed no correlations with the commonly used markers of kidney disease such as creatinine, urea, or symmetrical dimethylarginine. Nevertheless, we encourage the further investigation of NAG in relation to CKD, taking the breed, sex and age of the patients into account.

• Klíčová slova
• enzyme activity, kidney disease, tubular marker, urinalysis, urine enzyme,
• Publikační typ
• časopisecké články MeSH