PURPOSE: Cancer initialization can be explained as a result of parasitic virus energy consumption leading to randomized genome chemical bonding. MATERIALS AND METHODS: Analysis of experimental data on cell-mediated immunity (CMI) containing about 12,000 cases of healthy humans, cancer patients and patients with precancerous cervical lesions disclosed that the specific cancer and the non-specific lactate dehydrogenase-elevating (LDH) virus antigen elicit similar responses. The specific antigen is effective only in cancer type of its origin but the non-specific antigen in all examined cancers. CMI results of CIN patients display both healthy and cancer state. The ribonucleic acid (RNA) of the LDH virus parasitizing on energy reduces the ratio of coherent/random oscillations. Decreased effect of coherent cellular electromagnetic field on bonding electrons in biological macromolecules leads to elevating probability of random genome reactions. RESULTS: Overlapping of wave functions in biological macromolecules depends on energy of the cellular electromagnetic field which supplies energy to bonding electrons for selective chemical bonds. CMI responses of cancer and LDH virus antigens in all examined healthy, precancerous and cancer cases point to energy mechanism in cancer initiation. CONCLUSIONS: Dependence of the rate of biochemical reactions on biological electromagnetic field explains yet unknown mechanism of genome mutation.
- MeSH
- chemické modely MeSH
- elektromagnetická pole * MeSH
- LDH virus fyziologie MeSH
- lidé MeSH
- modely genetické MeSH
- modely imunologické MeSH
- mutace genetika účinky záření MeSH
- nádory genetika imunologie virologie MeSH
- onkogeny genetika imunologie účinky záření MeSH
- počítačová simulace MeSH
- T-lymfocyty imunologie účinky záření virologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Human and animal diseases are brought about by pathological alterations of production, composition, and conformation of macromolecules and structures in cells. Additional contributing factors include changes in physiological states caused by disturbances of energy supply, energy transduction, energy dissipation in moving or oscillating parts, and parasitic energy consumption. Disturbances of energy states may endanger existence of the system. The cell-mediated immunity (CMI) response of T lymphocytes correlating with their adherence properties was examined using antigen prepared from the serum of inbred laboratory mice strain C3H H(2k) infected with lactate dehydrogenase elevating (LDH) virus. LDH virus is a parasite on the cellular energy system. Significant CMI response was elicited in T lymphocytes prepared from the blood of patients with cancer of different phenotypes, acute myocardial infarctions, schizophrenia, and recurrent spontaneous abortions in early pregnancy from unknown reasons. The CMI response is assumed to monitor transferred information about decreased levels of energy states and decoherence in the cells caused by mitochondrial malfunction, parasitic consumption, production of lactate, and possibly other disturbances. The LDH virus infection or similar pathological processes caused by different agents might be connected with the diseases and monitored by the examined CMI response. A large amount of mitoses with chromosome defects in aborted fetuses suggest increased mutability of genomes caused by defective energy states.
- MeSH
- buněčná imunita MeSH
- energetický metabolismus * MeSH
- LDH virus fyziologie MeSH
- lidé MeSH
- myši MeSH
- nemoc * MeSH
- T-lymfocyty imunologie MeSH
- těhotenství MeSH
- viabilita buněk MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Cell-mediated immunity (CMI) response to different antigens was examined in healthy women, in patients with cervical precancerous lesions, and in patients with cervical cancer. Cervical lesions were diagnosed by cytological (PAP) smears, from examination by colposcopy, and from "punch" biopsy material by histology. CMI response is related to specific processes in healthy and cancer cells. CMI was investigated by leukocyte adherence inhibition (LAI) assay using specific antigen (prepared from cervical carcinoma tissue) and non specific antigen (prepared from blood of mice infected by LDH--lactate dehydrogenase--virus). The CMI responses of healthy women and cancer patients to the antigens used are different: the majority of T lymphocytes display adherence and non adherence, respectively (but the CMI responses elicited by the antigens are not equal and small quantitative differences are observed). Regardless of the CIN (cervical intraepithelial neoplasia) grades, CMI responses correspond either to healthy women or to cervical carcinoma patients (at about similar ratio of cases in all the CIN groups). Effect of non specific antigen suggests that cervical carcinoma transformation may be connected with reduction of mitochondrial activity similar to processes in LDH virus infection.
- MeSH
- antigeny nádorové metabolismus MeSH
- buněčná adheze MeSH
- buněčná imunita MeSH
- L-laktátdehydrogenasa metabolismus MeSH
- LDH virus metabolismus MeSH
- leukocyty cytologie MeSH
- lidé MeSH
- mitochondrie metabolismus MeSH
- nádory děložního čípku genetika imunologie patologie MeSH
- prekancerózy MeSH
- T-lymfocyty patologie virologie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
The authors were monitoring adherence ability of T lymphocytes in vitro in patients with laryngeal and pharyngeal carcinoma at the presence of tumor-specific and viral LDH antigen. The results were assessed and expressed in percent of non adherent T lymphocytes (NAL). First, NAL in patients before initiating the treatment was compared with NAL control group (voluntary blood donors). The ability of the adherence in T lymphocytes in the control group is statistically significantly higher. Further on, NAL in the course of a successful oncological treatment was monitored at the interval of 6 months following the treatment, and further on at yearly intervals. NAL level drops statistically significantly within 6 months and then hold on at levels with no statistical difference unlike the control group, however, the ability of T lymphocyte in patients to adhere remains statistically significantly lower. Statistically significantly higher levels of NAL are at the presence of LDH viral antigen. Further on, the authors were following the influence of magnetic sinusoidal field of power frequency (50 Hz) of a low induction (0.5, 0.1, and 0.05 mT) on NAL. NAL values under the influence of an experimental magnetic field before initiating the treatment as well as in the course of a successful oncological treatment are statistically significantly lower. It means that magnetic filed increases the adherence ability of T lymphocytes in patients with laryngeal and pharyngeal cancer in vitro.
- MeSH
- antigeny virové imunologie MeSH
- buněčná adheze účinky záření MeSH
- časové faktory MeSH
- LDH virus imunologie MeSH
- leukocyty patologie účinky záření MeSH
- lidé MeSH
- magnetismus MeSH
- myši MeSH
- nádory hlavy a krku imunologie patologie terapie MeSH
- nádory hltanu imunologie patologie terapie MeSH
- nádory hrtanu imunologie patologie terapie MeSH
- studie případů a kontrol MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- MeSH
- dospělí MeSH
- gelová chromatografie MeSH
- imunologická odpověď na dávku MeSH
- LDH virus patogenita MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední C3H MeSH
- myši MeSH
- nádory prsu diagnóza imunologie MeSH
- specificita protilátek krev MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- přehledy MeSH
- srovnávací studie MeSH
- MeSH
- alergologie a imunologie MeSH
- antigeny virové MeSH
- LDH virus imunologie MeSH
- lidé MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- MeSH
- buněčná imunita MeSH
- LDH virus MeSH
- lidé MeSH
- longitudinální studie MeSH
- nádory prsu imunologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- MeSH
- LDH virus genetika MeSH
- lidé MeSH
- longitudinální studie MeSH
- otorinolaryngologické nádory imunologie MeSH
- Check Tag
- lidé MeSH
