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Biodegradation of chlorpyrifos by Klebsiella sp. isolated from an activated sludge sample of waste water treatment plant in Damascus
Ghanem I, Orfi M, Shamma M.
Jazyk angličtina Země Česko
- MeSH
- biodegradace MeSH
- chromatografie plynová metody využití MeSH
- čištění vody metody MeSH
- dursban izolace a purifikace škodlivé účinky toxicita MeSH
- interpretace statistických dat MeSH
- Klebsiella chemie metabolismus růst a vývoj MeSH
- odpadní vody chemie mikrobiologie škodlivé účinky MeSH
A chlorpyrifos (CPY)-degrading bacterial strain was isolated from an activated sludge sample collected from the Damascus Wastewater Treatment Plant, Syria. The isolation of Klebsiella sp. was facilitated by the addition of CPY at a rate of 3.84 g/L of sludge weekly (selection pressure). Identification of Klebsiella sp. was done using major staining and biochemical differentiation tests (Gram stain, cytochrome oxidase and some relevant saccharide fermentation tests using biochemical assays). Klebsiella sp. was maintained by culturing in a poor medium consisting of mineral salts and CPY as the sole carbon source. When 3 activated sludge samples were incubated in the presence of CPY (13.9 g/L sludge), 46% of added CPY were degraded within 4 d. By comparison, within 4 d the isolated Klebsiella sp. was found to break down 92% of CPY when co-incubated in a poor mineral medium in which CPY was the sole carbon source (13.9 g/L poor medium). Isolated Klebsiella sp. was able to tolerate up to 17.3 g of CPY in the poor medium.
Lit.: 21
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- $a Biodegradation of chlorpyrifos by Klebsiella sp. isolated from an activated sludge sample of waste water treatment plant in Damascus. / $c Ghanem I, Orfi M, Shamma M.
- 314 __
- $a Biotechnology Department, Atomic Energy Commission of Syria, Damascus
- 504 __
- $a Lit.: 21
- 520 9_
- $a A chlorpyrifos (CPY)-degrading bacterial strain was isolated from an activated sludge sample collected from the Damascus Wastewater Treatment Plant, Syria. The isolation of Klebsiella sp. was facilitated by the addition of CPY at a rate of 3.84 g/L of sludge weekly (selection pressure). Identification of Klebsiella sp. was done using major staining and biochemical differentiation tests (Gram stain, cytochrome oxidase and some relevant saccharide fermentation tests using biochemical assays). Klebsiella sp. was maintained by culturing in a poor medium consisting of mineral salts and CPY as the sole carbon source. When 3 activated sludge samples were incubated in the presence of CPY (13.9 g/L sludge), 46% of added CPY were degraded within 4 d. By comparison, within 4 d the isolated Klebsiella sp. was found to break down 92% of CPY when co-incubated in a poor mineral medium in which CPY was the sole carbon source (13.9 g/L poor medium). Isolated Klebsiella sp. was able to tolerate up to 17.3 g of CPY in the poor medium.
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