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Hybridization analysis and mapping of the celesticetin gene cluster revealed genes shared with lincomycin biosynthesis
L. Čermák, J. Novotná, M. Ságová-Marečková, J. Kopecký, L. Najmanová, J. Janata
Jazyk angličtina Země Česko
- MeSH
- digoxigenin analogy a deriváty MeSH
- DNA bakterií genetika MeSH
- finanční podpora výzkumu jako téma MeSH
- hybridizace genetická genetika imunologie MeSH
- léková rezistence genetika imunologie MeSH
- linkomycin analogy a deriváty analýza chemie MeSH
- mapování chromozomů metody MeSH
- otevřené čtecí rámce genetika MeSH
- polymerázová řetězová reakce metody využití MeSH
- regulace genové exprese u bakterií MeSH
- Southernův blotting metody využití MeSH
- Streptomyces coelicolor enzymologie genetika MeSH
- Streptomyces enzymologie genetika MeSH
The first insight into celesticetin biosynthetic gene cluster of S. caelestis is presented. The genomic DNA of producing strain was digested, digoxigenin-labeled and hybridized with a set of probes designed according to S. lincolnensis gene sequences. Genes with high homology to the lincomycin biosynthetic genes coding for the predicted common parts of the pathway were identified in S. caelestis. Then, genomic DNA of S. caelestis treated by a multiple digestion was hybridized with five digoxigenin-labeled probes to construct a rough restriction map. Two consecutive islands formed by the genes with a putative function in biosynthesis of the shared saccharide moiety revealed an organization similar to the lincomycin biosynthetic gene cluster. The celesticetin cluster was mapped and essential information was obtained for subsequent steps, i.e. isolation and sequence analysis of the cluster.
Lit.: 16
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- $a Institute of Microbiology, Prague
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- $a Lit.: 16
- 520 9_
- $a The first insight into celesticetin biosynthetic gene cluster of S. caelestis is presented. The genomic DNA of producing strain was digested, digoxigenin-labeled and hybridized with a set of probes designed according to S. lincolnensis gene sequences. Genes with high homology to the lincomycin biosynthetic genes coding for the predicted common parts of the pathway were identified in S. caelestis. Then, genomic DNA of S. caelestis treated by a multiple digestion was hybridized with five digoxigenin-labeled probes to construct a rough restriction map. Two consecutive islands formed by the genes with a putative function in biosynthesis of the shared saccharide moiety revealed an organization similar to the lincomycin biosynthetic gene cluster. The celesticetin cluster was mapped and essential information was obtained for subsequent steps, i.e. isolation and sequence analysis of the cluster.
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