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RAPD and rep-PCR fingerprinting for characterization of Bifidobacterium species
J. Křížová, Alena Španová, Bohuslav Rittich
Jazyk angličtina Země Česko
- MeSH
- Bifidobacterium genetika izolace a purifikace klasifikace MeSH
- DNA bakterií genetika MeSH
- DNA fingerprinting metody MeSH
- fylogeneze MeSH
- lidé MeSH
- polymerázová řetězová reakce metody MeSH
- technika náhodné amplifikace polymorfní DNA metody MeSH
- techniky typizace bakterií metody MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
DNA fingerprinting methods, RAPD with 7 random primers, and rep-PCR using both BOXA1R and (GTG)(5) ones, were used for the discrimination of 16 type and collection Bifidobacterium strains of 9 species of human origin, B. animalis ssp. animalis and B. animalis ssp. lactis and 7 Bifidobacterium strains collected in the Culture Collection of Dairy Microorganisms (CCDM). Both RAPD and rep-PCR methods provided similar results. The strains were identified as B. animalis ssp. lactis (6 strains) and B. adolescentis (1 strain). The reclassification of the collection strain CCM 3761 as B. pseudocatenulatum species (previously classified as B. adolescentis) was confirmed.
Lit.: 26
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- $2 doi $a 10.1007/s12223-008-0014-1
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- $a Lit.: 26
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- $a DNA fingerprinting methods, RAPD with 7 random primers, and rep-PCR using both BOXA1R and (GTG)(5) ones, were used for the discrimination of 16 type and collection Bifidobacterium strains of 9 species of human origin, B. animalis ssp. animalis and B. animalis ssp. lactis and 7 Bifidobacterium strains collected in the Culture Collection of Dairy Microorganisms (CCDM). Both RAPD and rep-PCR methods provided similar results. The strains were identified as B. animalis ssp. lactis (6 strains) and B. adolescentis (1 strain). The reclassification of the collection strain CCM 3761 as B. pseudocatenulatum species (previously classified as B. adolescentis) was confirmed.
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