-
Je něco špatně v tomto záznamu ?
Heterologous expression of mammalian Na/H antiporters in Saccharomyces cerevisiae
Flegelova H., Haguenauer-Tsapis R., Sychrova H.
Jazyk angličtina Země Nizozemsko
- MeSH
- fenotyp MeSH
- financování organizované MeSH
- glycerol farmakologie MeSH
- klonování DNA MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- mutantní chimérické proteiny fyziologie MeSH
- Na(+)-H(+) antiport biosyntéza genetika MeSH
- rekombinantní proteiny biosyntéza MeSH
- Saccharomyces cerevisiae genetika metabolismus účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
Na+/H+ antiporters, integral membrane proteins that exchange protons for alkali metal cations, play multiple roles in probably all living organisms (preventing cells from excessive amounts of alkali metal cations, regulating intracellular pH and cell volume). In this work, we studied the functionality of rat plasma membrane NHE1-3 exchangers upon their heterologous expression in alkali-metal-cation sensitive Saccharomyces cerevisiae, and searched for conditions that would increase their level in the plasma membrane and improve their functionality. Though three tested exchangers were partially localized to the plasma membrane (and two of them (NHE2 and NHE3) in an active form), the bulk of the synthesized proteins were arrested along the secretory pathway, mainly in the ER. To increase the level of exchangers in the yeast plasma membrane several approaches (truncation of C-terminal regulatory sequences, expression in mutant yeast strains, construction of rat/yeast protein chimeras, various growth conditions and chemical chaperones) were tested. The only increase in the amount of NHE exchangers in the plasma membrane was obtained upon expression in a strain with the npi1 mutation, which significantly lowers the level of Rsp5 ubiquitin ligase in cells. This mutation helped to stabilize proteins in the plasma membrane.
- 000
- 00000naa 2200000 a 4500
- 001
- bmc07521327
- 003
- CZ-PrNML
- 005
- 20120812220134.0
- 008
- 090415s2006 ne e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Flegelová, Hana $7 xx0118984
- 245 10
- $a Heterologous expression of mammalian Na/H antiporters in Saccharomyces cerevisiae / $c Flegelova H., Haguenauer-Tsapis R., Sychrova H.
- 314 __
- $a Department of Membrane Transport, Institute of Physiology AS CR, Videnska 1083, 142 20, Prague 4, Czech Republic.
- 520 9_
- $a Na+/H+ antiporters, integral membrane proteins that exchange protons for alkali metal cations, play multiple roles in probably all living organisms (preventing cells from excessive amounts of alkali metal cations, regulating intracellular pH and cell volume). In this work, we studied the functionality of rat plasma membrane NHE1-3 exchangers upon their heterologous expression in alkali-metal-cation sensitive Saccharomyces cerevisiae, and searched for conditions that would increase their level in the plasma membrane and improve their functionality. Though three tested exchangers were partially localized to the plasma membrane (and two of them (NHE2 and NHE3) in an active form), the bulk of the synthesized proteins were arrested along the secretory pathway, mainly in the ER. To increase the level of exchangers in the yeast plasma membrane several approaches (truncation of C-terminal regulatory sequences, expression in mutant yeast strains, construction of rat/yeast protein chimeras, various growth conditions and chemical chaperones) were tested. The only increase in the amount of NHE exchangers in the plasma membrane was obtained upon expression in a strain with the npi1 mutation, which significantly lowers the level of Rsp5 ubiquitin ligase in cells. This mutation helped to stabilize proteins in the plasma membrane.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a klonování DNA $7 D003001
- 650 _2
- $a glycerol $x farmakologie $7 D005990
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mutantní chimérické proteiny $x fyziologie $7 D050506
- 650 _2
- $a fenotyp $7 D010641
- 650 _2
- $a krysa rodu Rattus $7 D051381
- 650 _2
- $a rekombinantní proteiny $x biosyntéza $7 D011994
- 650 _2
- $a Saccharomyces cerevisiae $x genetika $x metabolismus $x účinky léků $7 D012441
- 650 _2
- $a Na(+)-H(+) antiport $x biosyntéza $x genetika $7 D017923
- 650 _2
- $a financování organizované $7 D005381
- 700 1_
- $a Haguenauer-Tsapis, Rosine
- 700 1_
- $a Sychrová, Hana, $d 1959- $7 xx0030590
- 773 0_
- $w MED00009314 $t Biochimica et biophysica acta. Complete edition $g Roč. 1760, č. 3 (2006), s. 504-516 $x 0006-3002
- 910 __
- $a ABA008 $b A 806 $y 9
- 990 __
- $a 20090312170439 $b ABA008
- 991 __
- $a 20120812220249 $b ABA008
- 999 __
- $a ok $b bmc $g 644167 $s 497048
- BAS __
- $a 3
- BMC __
- $a 2006 $b 1760 $c 3 $d 504-516 $i 0006-3002 $m Biochimica et biophysica acta $x MED00009314
- LZP __
- $a 2009-B1/vtme