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Yarrowia lipolytica possesses two plasma membrane alkali metal cation/H+ antiporters with different functions in cell physiology
Papouskova K, Sychrova H.
Jazyk angličtina Země Nizozemsko
E-zdroje NLK Online
Free Medical Journals od 1968 do 2015ScienceDirect (archiv) od 1993-01-01 do 2009-12-31
Wiley Free Content od 1997 do Před 1 rokem
Elsevier Open Access Journals od 1968-07-01 do 2015-12-21
Elsevier Open Archive Journals od 1968-07-01 do Před 1 rokem
- MeSH
- antiportéry metabolismus MeSH
- buněčná membrána metabolismus MeSH
- časové faktory MeSH
- draslík metabolismus MeSH
- exprese genu MeSH
- financování organizované MeSH
- fungální proteiny metabolismus MeSH
- rekombinantní fúzní proteiny metabolismus MeSH
- Saccharomyces cerevisiae cytologie MeSH
- sekvenční analýza proteinů MeSH
- sodík metabolismus MeSH
- soli farmakologie MeSH
- transport proteinů MeSH
- Yarrowia fyziologie metabolismus účinky léků MeSH
The family of Nha antiporters mediating the efflux of alkali metal cations in exchange for protons across the plasma membrane is conserved in all yeast species. Yarrowia lipolytica is a dimorphic yeast, phylogenetically very distant from the model yeast Saccharomyces cerevisiae. A search in its sequenced genome revealed two genes (designated as YlNHA1 and YlNHA2) with homology to the S. cerevisiae NHA1 gene, which encodes a plasma membrane alkali metal cation/H+ antiporter. Upon heterologous expression of both YlNHA genes in S. cerevisiae, we showed that Y. lipolytica antiporters differ not only in length and sequence, but also in their affinity for individual substrates. While the YlNha1 protein mainly increased cell tolerance to potassium, YlNha2p displayed a remarkable transport capacity for sodium. Thus, Y. lipolytica is the first example of a yeast species with two plasma membrane alkali metal cation/H+ antiporters differing in their putative functions in cell physiology; cell detoxification vs. the maintenance of stable intracellular pH, potassium content and cell volume.
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- $a The family of Nha antiporters mediating the efflux of alkali metal cations in exchange for protons across the plasma membrane is conserved in all yeast species. Yarrowia lipolytica is a dimorphic yeast, phylogenetically very distant from the model yeast Saccharomyces cerevisiae. A search in its sequenced genome revealed two genes (designated as YlNHA1 and YlNHA2) with homology to the S. cerevisiae NHA1 gene, which encodes a plasma membrane alkali metal cation/H+ antiporter. Upon heterologous expression of both YlNHA genes in S. cerevisiae, we showed that Y. lipolytica antiporters differ not only in length and sequence, but also in their affinity for individual substrates. While the YlNha1 protein mainly increased cell tolerance to potassium, YlNha2p displayed a remarkable transport capacity for sodium. Thus, Y. lipolytica is the first example of a yeast species with two plasma membrane alkali metal cation/H+ antiporters differing in their putative functions in cell physiology; cell detoxification vs. the maintenance of stable intracellular pH, potassium content and cell volume.
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