• Something wrong with this record ?

Sequence analysis and heterologous expression of the lincomycin biosynthetic cluster of the type strain Streptomyces lincolnensis ATCC 25466

Markéta Koběrská, Jan Kopecký, Jana Olšovská, Markéta Jelínková, Dana Ulanová, Petr Man, Miroslav Flieger, Jiří Janata

. 2008 ; 53 (5) : 395-401.

Language English Country Czech Republic

Persistent link   https://www.medvik.cz/link/bmc07523238

A cosmid bearing an insert of 38 217 bp covering the gene cluster and its flanking regions of type strain Streptomyces lincolnensis ATCC 25466 was sequenced. Two relatively extensive sequence changes and several hundred point mutations were identified if compared with the previously published sequence of the lincomycin (Lin) industrial strain S. lincolnensis 78-11. Analysis of the cluster-flanking regions revealed its localization within the genome of the ATCC 25466 strain. The cluster-bearing cosmid was integrated into the chromosome of Lin non-producing strains S. coelicolor CH 999 and S. coelicolor M 145. The modified strains heterologously produced Lin but the level dropped to approximately 1-3% of the production in the ATCC 25466 strain.

Bibliography, etc.

Lit.: 23

000      
00000naa 2200000 a 4500
001      
bmc07523238
003      
CZ-PrNML
005      
20200925090309.0
008      
090514s2008 xr e eng||
009      
AR
024    7_
$2 doi $a 10.1007/s12223-008-0060-8
040    __
$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Koběrská, Markéta. $7 _AN032056
245    10
$a Sequence analysis and heterologous expression of the lincomycin biosynthetic cluster of the type strain Streptomyces lincolnensis ATCC 25466 / $c Markéta Koběrská, Jan Kopecký, Jana Olšovská, Markéta Jelínková, Dana Ulanová, Petr Man, Miroslav Flieger, Jiří Janata
314    __
$a Institute of Microbiology of the Academy of Sciences of the Czech Republic, v.v.i., Prague
504    __
$a Lit.: 23
520    9_
$a A cosmid bearing an insert of 38 217 bp covering the gene cluster and its flanking regions of type strain Streptomyces lincolnensis ATCC 25466 was sequenced. Two relatively extensive sequence changes and several hundred point mutations were identified if compared with the previously published sequence of the lincomycin (Lin) industrial strain S. lincolnensis 78-11. Analysis of the cluster-flanking regions revealed its localization within the genome of the ATCC 25466 strain. The cluster-bearing cosmid was integrated into the chromosome of Lin non-producing strains S. coelicolor CH 999 and S. coelicolor M 145. The modified strains heterologously produced Lin but the level dropped to approximately 1-3% of the production in the ATCC 25466 strain.
650    _2
$a antibakteriální látky $x biosyntéza $x chemie $7 D000900
650    _2
$a bakteriální proteiny $x genetika $x metabolismus $7 D001426
650    _2
$a biotechnologie $7 D001709
650    _2
$a kosmidy $7 D003360
650    _2
$a genová knihovna $7 D015723
650    _2
$a linkomycin $x biosyntéza $x chemie $7 D008034
650    _2
$a multigenová rodina $7 D005810
650    _2
$a bodová mutace $7 D017354
650    _2
$a sekvenční analýza DNA $7 D017422
650    _2
$a Streptomyces $x genetika $x metabolismus $x růst a vývoj $7 D013302
650    _2
$a Streptomyces coelicolor $x genetika $x metabolismus $7 D048372
700    1_
$a Kopecký, Jan, $d 1966- $7 xx0108100
700    1_
$a Olšovská, Jana $7 xx0096915
700    1_
$a Jelínková-Ibrahimová, Markéta $7 xx0096917
700    1_
$a Ulanová, Dana $7 xx0096916
700    1_
$a Man, Petr $7 xx0131981
700    1_
$a Flieger, Miroslav, $d 1948-
700    1_
$a Janata, Jiří $7 xx0114152
773    0_
$w MED00011005 $t Folia microbiologica $g Roč. 53, č. 5 (2008), s. 395-401 $x 0015-5632
910    __
$a ABA008 $b A 2403 $c 561 $y 9 $z 0
990    __
$a 20090514125508 $b ABA008
991    __
$a 20200925090307 $b ABA008
999    __
$a ok $b bmc $g 651326 $s 504498
BAS    __
$a 3
BMC    __
$a 2008 $b 53 $c 5 $d 395-401 $i 0015-5632 $m Folia microbiologica $x MED00011005
LZP    __
$a 2009-18/mkme

Find record

Citation metrics

Archiving options