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Production of polyclonal antibodies to a recombinant coat protein of potato virus Y
Joanna Folwarczna, Helena Plchová, Tomáš Moravec, Hana Hoffmeisterová, Petr Dědič, Noemi Čeřovská
Jazyk angličtina Země Česko
- MeSH
- ELISA MeSH
- králíci MeSH
- monoklonální protilátky biosyntéza MeSH
- nemoci rostlin virologie MeSH
- Potyvirus imunologie MeSH
- protilátky virové biosyntéza MeSH
- rekombinantní proteiny imunologie MeSH
- Solanum tuberosum virologie MeSH
- tabák virologie MeSH
- virové plášťové proteiny imunologie MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laboratory host Nicotiana tabacum cv. Petit Havana SR1 and in various cultivars of the natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies can be used for the detection of the whole strain spectrum of PVY by indirect plate trapped antigen ELISA and Western blot, but not by double antigen sandwich ELISA.
Lit.: 21
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- 314 __
- $a Intitute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague
- 504 __
- $a Lit.: 21
- 520 9_
- $a The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laboratory host Nicotiana tabacum cv. Petit Havana SR1 and in various cultivars of the natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies can be used for the detection of the whole strain spectrum of PVY by indirect plate trapped antigen ELISA and Western blot, but not by double antigen sandwich ELISA.
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- $a monoklonální protilátky $x biosyntéza $7 D000911
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- $a protilátky virové $x biosyntéza $7 D000914
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- $a Solanum tuberosum $x virologie $7 D011198
- 650 _2
- $a tabák $x virologie $7 D014026
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- $a Plchová, Helena $7 xx0096918
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- $a Moravec, Tomáš $7 xx0096749
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- $a Hoffmeisterová, Hana $7 xx0096919
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