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Molecular characterization of intervening sequences in 23S rRNA genes and 23S rRNA fragmentation in Taylorella equigenitalis
A. Tazumi, T. Sekizuka, J. E. Moore, B. C. Millar, I. Taneike, M. Matsuda
Language English Country Czech Republic
- MeSH
- Genes, Bacterial MeSH
- RNA, Bacterial genetics MeSH
- Species Specificity MeSH
- Gram-Negative Bacterial Infections microbiology veterinary MeSH
- Introns genetics MeSH
- Horses MeSH
- Nucleic Acid Conformation MeSH
- Consensus Sequence MeSH
- Molecular Sequence Data MeSH
- Horse Diseases microbiology MeSH
- RNA Processing, Post-Transcriptional MeSH
- RNA, Ribosomal, 23S genetics MeSH
- Base Sequence MeSH
- Sequence Homology, Nucleic Acid MeSH
- Sequence Alignment MeSH
- Taylorella equigenitalis genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
Using two primer pairs constructed in silico for the amplification of the intervening sequences (IVSs) of the 23S rRNA gene sequences of the genus Taylorella, none of the three representative T. equigenitalis strains NCTC11184(T), Kentucky 188 and EQ59 was shown to contain any IVSs in the first quarter region. In the central region, all three strains possessed one approximately 70 bp IVS (TeIVS2) different from any IVSs found in T. asinigenitalis. The predicted secondary structure model of the IVSs contained stem and loop structures. The central region of the IVS-stem structure contains an identical double-stranded consensus 15-bp sequence. The purified RNA fraction from the three strains contained 16S and 4-5S RNA species but no 23S rRNA species. Thus, the primary 23S rRNA transcripts from the three strains would be cleaved into approximately 1.2- and 1.6-kb rRNA fragments and approximately 70-bp IVS. In addition, 16 other T. equigenitalis isolates were found to carry a similar 70-bp IVS in the central region and to produce fragmented 23S rRNA.
Lit.: 32
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- 20111210135456.0
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- 090519s2008 xr e eng||
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- $2 doi $a 10.1007/s12223-008-0076-0
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- $a Tazumi, A.
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- $a Molecular characterization of intervening sequences in 23S rRNA genes and 23S rRNA fragmentation in Taylorella equigenitalis / $c A. Tazumi, T. Sekizuka, J. E. Moore, B. C. Millar, I. Taneike, M. Matsuda
- 314 __
- $a Azabu University, Fuchinobe, Sagamihara
- 504 __
- $a Lit.: 32
- 520 9_
- $a Using two primer pairs constructed in silico for the amplification of the intervening sequences (IVSs) of the 23S rRNA gene sequences of the genus Taylorella, none of the three representative T. equigenitalis strains NCTC11184(T), Kentucky 188 and EQ59 was shown to contain any IVSs in the first quarter region. In the central region, all three strains possessed one approximately 70 bp IVS (TeIVS2) different from any IVSs found in T. asinigenitalis. The predicted secondary structure model of the IVSs contained stem and loop structures. The central region of the IVS-stem structure contains an identical double-stranded consensus 15-bp sequence. The purified RNA fraction from the three strains contained 16S and 4-5S RNA species but no 23S rRNA species. Thus, the primary 23S rRNA transcripts from the three strains would be cleaved into approximately 1.2- and 1.6-kb rRNA fragments and approximately 70-bp IVS. In addition, 16 other T. equigenitalis isolates were found to carry a similar 70-bp IVS in the central region and to produce fragmented 23S rRNA.
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