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Vývoj neinvazivní diagnostiky Asthma bronchiale
[Development of a non-invasive diagnostic method for bronchial asthma]
K. Syslová, J. Přech, J. Lebedová, D. Pelclová, P. Kačera
Jazyk čeština Země Česko
- MeSH
- bronchiální astma diagnóza MeSH
- dechové testy metody MeSH
- hmotnostní spektrometrie metody MeSH
- imunosorpční techniky MeSH
- leukotrieny analýza MeSH
- SRS-A analýza MeSH
A method was developed for rapid and accurate determination of cysteinylleukotrienes (LTC4, LTD4, LTE4), essential biomarkers of bronchial asthma, in exhaled breath condensate. The method consists of an immunoseparation procedure combined with selective and sensitive detection by LC-ESI-MS in the multiple reaction monitoring mode (MRM) and using a stable-isotopedilution assay. The precision of the method was around 94 %. The mean accuracy was higher than 90 % for the content of cysteinylleukotrienes in the concentrate up to 250 pg ml-1. The method was tested on patients with occupational bronchial asthma and compared with healthy subjects. The differences between the two groups were significant.
Development of a non-invasive diagnostic method for bronchial asthma
Lit.: 15
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- $a Ústav organické technologie, Vysoká škola chemicko-technologická, Praha
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- $a Lit.: 15
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- $a A method was developed for rapid and accurate determination of cysteinylleukotrienes (LTC4, LTD4, LTE4), essential biomarkers of bronchial asthma, in exhaled breath condensate. The method consists of an immunoseparation procedure combined with selective and sensitive detection by LC-ESI-MS in the multiple reaction monitoring mode (MRM) and using a stable-isotopedilution assay. The precision of the method was around 94 %. The mean accuracy was higher than 90 % for the content of cysteinylleukotrienes in the concentrate up to 250 pg ml-1. The method was tested on patients with occupational bronchial asthma and compared with healthy subjects. The differences between the two groups were significant.
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