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Effect of lithium on smooth muscle contraction and phosphorylation of myosin light chain by MLCK
Z. Y. Tang, Z. N. Liu, L. Fu, D. P. Chen, Q. D. Ai, Y. Lin
Language English Country Czech Republic
NLK
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- MeSH
- Financing, Organized MeSH
- Phosphorylation MeSH
- Muscle, Smooth metabolism drug effects MeSH
- Myosin-Light-Chain Kinase metabolism MeSH
- Rabbits MeSH
- Myosin Light Chains metabolism MeSH
- Lithium pharmacology MeSH
- Muscle Contraction drug effects MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Animals MeSH
The aims of our study were to investigate into the effect of lithium on smooth muscle contraction and phosphorylation of myosin light chain (MLC20) by MLCK and to find out the clue of its mechanism. Isolated rabbit duodenum smooth muscle strips were used to study the effects of lithium on their contractile activity under the condition of Krebs' solution by means of HW- 400S constant temperature smooth muscle trough. Myosin and MLCK were purified from the chicken gizzard smooth muscle. Myosin phosphorylation was determined by Glycerol-PAGE, myosin Mg2+-ATPase activity was measured by Pi liberation method. Lithium (10-40 mM) inhibited the contraction in duodenum in a dose-related and time-dependent manner. Lithium could also inhibit the extent of myosin phosphorylation in a dose-related and time-dependent manner, whereas it inhibited Mg2+-ATPase activity in a dose-related manner. Lithium inhibited smooth muscle contraction by inhibition of myosin phosphorylation and Mg2+-ATPase activity.
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Lit.: 36
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- $a Department of Pharmacology, Dalian Medical University, Dalian
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- $a Lit.: 36
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- $a The aims of our study were to investigate into the effect of lithium on smooth muscle contraction and phosphorylation of myosin light chain (MLC20) by MLCK and to find out the clue of its mechanism. Isolated rabbit duodenum smooth muscle strips were used to study the effects of lithium on their contractile activity under the condition of Krebs' solution by means of HW- 400S constant temperature smooth muscle trough. Myosin and MLCK were purified from the chicken gizzard smooth muscle. Myosin phosphorylation was determined by Glycerol-PAGE, myosin Mg2+-ATPase activity was measured by Pi liberation method. Lithium (10-40 mM) inhibited the contraction in duodenum in a dose-related and time-dependent manner. Lithium could also inhibit the extent of myosin phosphorylation in a dose-related and time-dependent manner, whereas it inhibited Mg2+-ATPase activity in a dose-related manner. Lithium inhibited smooth muscle contraction by inhibition of myosin phosphorylation and Mg2+-ATPase activity.
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