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Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems
V. Chromy, K. Rozkosna, P. Sedlak
Jazyk angličtina Země Německo
- MeSH
- artefakty MeSH
- bilirubin metabolismus MeSH
- biochemická analýza krve metody normy MeSH
- časové faktory MeSH
- dítě MeSH
- dospělí MeSH
- financování organizované MeSH
- kalibrace MeSH
- kinetika MeSH
- kreatinin krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- oxidace-redukce MeSH
- referenční standardy MeSH
- senioři MeSH
- tartaráty metabolismus MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
BACKGROUND: The calibration of Jaffe method for serum creatinine using one serum-based standard complemented with artificial matrix compensating standard's Jaffe-interfering substances allows two-point calibration with results well comparable with enzymatic methods. METHOD: Spectrophotometry was used. RESULTS: Jaffe procedures compensating serum/plasma intereferents by subtracting a constant amount of creatinine poorly overcompensate creatinine in children. Two-point calibration with a pair of primary serum standards certified by the reference measurement procedure (isotope-dilution, mass spectrometry) or with a pair of secondary standards linked to primary materials could provide results well agreeable with enzymatic determination. Such a calibration comprises an absorbance offset corresponding to other-than-creatinine Jaffe-interfering chromogens present in standards in the calibration line, while a two-point calibration combining one standard with physiological saline/water always grossly distorts calibration line. We calculated/prepared artificial serum matrices capable of compensating Jaffe-interfering chromogens in serum standards. The combination of even one standard with its artificial matrix also enables two-point calibration with practically the same results as with a pair of primary standards. CONCLUSIONS: A two-point calibration of Jaffe method for serum creatinine combining only one serum standard with creatinine solution matching standard's allow matrix-present interferents present results well comparable with enzymatic determination, providing the standard is attested/linked to the reference measurement procedure.
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- 20121030115143.0
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- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
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- $a Chromý, Vratislav, $d 1937- $7 jn20010309815
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- $a Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems / $c V. Chromy, K. Rozkosna, P. Sedlak
- 314 __
- $a Institute of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic. vrch@chemi.muni.cz
- 520 9_
- $a BACKGROUND: The calibration of Jaffe method for serum creatinine using one serum-based standard complemented with artificial matrix compensating standard's Jaffe-interfering substances allows two-point calibration with results well comparable with enzymatic methods. METHOD: Spectrophotometry was used. RESULTS: Jaffe procedures compensating serum/plasma intereferents by subtracting a constant amount of creatinine poorly overcompensate creatinine in children. Two-point calibration with a pair of primary serum standards certified by the reference measurement procedure (isotope-dilution, mass spectrometry) or with a pair of secondary standards linked to primary materials could provide results well agreeable with enzymatic determination. Such a calibration comprises an absorbance offset corresponding to other-than-creatinine Jaffe-interfering chromogens present in standards in the calibration line, while a two-point calibration combining one standard with physiological saline/water always grossly distorts calibration line. We calculated/prepared artificial serum matrices capable of compensating Jaffe-interfering chromogens in serum standards. The combination of even one standard with its artificial matrix also enables two-point calibration with practically the same results as with a pair of primary standards. CONCLUSIONS: A two-point calibration of Jaffe method for serum creatinine combining only one serum standard with creatinine solution matching standard's allow matrix-present interferents present results well comparable with enzymatic determination, providing the standard is attested/linked to the reference measurement procedure.
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