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Premature processing of mouse mammary tumor virus Gag polyprotein impairs intracellular capsid assembly
A Zabransky, R Hadravova, J Stokrova, M Sakalian, I Pichova
Language English Country United States
NLK
ScienceDirect (archiv)
from 1993-01-01 to 2009-12-31
Elsevier Open Access Journals
from 1995-01-10 to 1 year ago
Elsevier Open Archive Journals
from 1995-01-10 to 1 year ago
- MeSH
- Dexamethasone pharmacology MeSH
- Mammary Neoplasms, Experimental virology MeSH
- Financing, Organized MeSH
- Gene Products, gag genetics metabolism MeSH
- Kinetics MeSH
- Humans MeSH
- Mammary Glands, Animal virology MeSH
- Mice MeSH
- Mammary Neoplasms, Animal virology MeSH
- Promoter Regions, Genetic MeSH
- Peptide Hydrolases genetics metabolism MeSH
- Proviruses genetics MeSH
- Restriction Mapping MeSH
- Amino Acid Substitution MeSH
- T-Lymphocytes drug effects virology MeSH
- Transfection MeSH
- Mammary Tumor Virus, Mouse genetics metabolism MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
Mouse mammary tumor virus (MMTV) is the prototypical member of the Betaretrovirus genus, but the processes of its morphogenesis are poorly characterized. In this report, we describe an unusual intracellular processing of MMTV Gag polyprotein in human 293T cells transiently expressing MMTV from heterologous promoter. The same specific cleavage products of the viral protease were seen for the wild type as well as for nonmyristylated mutant of MMTV Gag polyprotein completely defective in the particle release. Inactivation of the viral protease resulted in more stable Gag polyprotein and in accumulation of intracytoplasmic particles for nonmyristylated Gag. The intracellular processing of nonmyristylated MMTV Gag indicates that protease activation in betaretrovirus can occur independently of budding.
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- $a Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic. ales@uochb.cas.cz
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- $a Mouse mammary tumor virus (MMTV) is the prototypical member of the Betaretrovirus genus, but the processes of its morphogenesis are poorly characterized. In this report, we describe an unusual intracellular processing of MMTV Gag polyprotein in human 293T cells transiently expressing MMTV from heterologous promoter. The same specific cleavage products of the viral protease were seen for the wild type as well as for nonmyristylated mutant of MMTV Gag polyprotein completely defective in the particle release. Inactivation of the viral protease resulted in more stable Gag polyprotein and in accumulation of intracytoplasmic particles for nonmyristylated Gag. The intracellular processing of nonmyristylated MMTV Gag indicates that protease activation in betaretrovirus can occur independently of budding.
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