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Premature processing of mouse mammary tumor virus Gag polyprotein impairs intracellular capsid assembly
A Zabransky, R Hadravova, J Stokrova, M Sakalian, I Pichova
Jazyk angličtina Země Spojené státy americké
NLK
ScienceDirect (archiv)
od 1993-01-01 do 2009-12-31
Elsevier Open Access Journals
od 1995-01-10 do Před 1 rokem
Elsevier Open Archive Journals
od 1995-01-10 do Před 1 rokem
- MeSH
- dexamethason farmakologie MeSH
- experimentální nádory mléčných žláz virologie MeSH
- financování organizované MeSH
- genové produkty gag genetika metabolismus MeSH
- kinetika MeSH
- lidé MeSH
- mléčné žlázy zvířat virologie MeSH
- myši MeSH
- nádory mléčné žlázy u zvířat virologie MeSH
- promotorové oblasti (genetika) MeSH
- proteasy genetika metabolismus MeSH
- proviry genetika MeSH
- restrikční mapování MeSH
- substituce aminokyselin MeSH
- T-lymfocyty účinky léků virologie MeSH
- transfekce MeSH
- virus myšího tumoru prsní žlázy genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
Mouse mammary tumor virus (MMTV) is the prototypical member of the Betaretrovirus genus, but the processes of its morphogenesis are poorly characterized. In this report, we describe an unusual intracellular processing of MMTV Gag polyprotein in human 293T cells transiently expressing MMTV from heterologous promoter. The same specific cleavage products of the viral protease were seen for the wild type as well as for nonmyristylated mutant of MMTV Gag polyprotein completely defective in the particle release. Inactivation of the viral protease resulted in more stable Gag polyprotein and in accumulation of intracytoplasmic particles for nonmyristylated Gag. The intracellular processing of nonmyristylated MMTV Gag indicates that protease activation in betaretrovirus can occur independently of budding.
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- $a Mouse mammary tumor virus (MMTV) is the prototypical member of the Betaretrovirus genus, but the processes of its morphogenesis are poorly characterized. In this report, we describe an unusual intracellular processing of MMTV Gag polyprotein in human 293T cells transiently expressing MMTV from heterologous promoter. The same specific cleavage products of the viral protease were seen for the wild type as well as for nonmyristylated mutant of MMTV Gag polyprotein completely defective in the particle release. Inactivation of the viral protease resulted in more stable Gag polyprotein and in accumulation of intracytoplasmic particles for nonmyristylated Gag. The intracellular processing of nonmyristylated MMTV Gag indicates that protease activation in betaretrovirus can occur independently of budding.
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