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Multiplex PCR assay for detection of Actinobacillus pleuropneumoniae, Pasteurella multocida and Haemophilus parasuis in lungs of pigs from a slaughterhouse
M. Hričínová, E. Holoda, D. Mudroňová, S. Ondrašovičová
Folia microbiologica.
2010 ;
55 (6) :
635-640.
Jazyk angličtina Země Česko
Typ dokumentu srovnávací studie
Perzistentní odkaz
https://www.medvik.cz/link/bmc12003961
- MeSH
- Actinobacillus pleuropneumoniae genetika izolace a purifikace MeSH
- bakteriologické techniky metody MeSH
- DNA primery MeSH
- financování organizované MeSH
- Haemophilus parasuis genetika izolace a purifikace MeSH
- hodnotící studie jako téma MeSH
- jatka MeSH
- Pasteurella multocida chemie izolace a purifikace MeSH
- plíce mikrobiologie MeSH
- polymerázová řetězová reakce metody MeSH
- prasata MeSH
- prevalence MeSH
- senzitivita a specificita MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
Multiplex PCR has been developed for parallel identification of Actinobacillus pleuropneumoniae, Pasteurella multocida and Haemophilus parasuis, important pathogens of swine, responsible for considerable economic losses in swine industry. Multiplex PCR and bacteriological cultivation was used to analyze lung samples from slaughterhouse pigs. From a total of 219 lung samples, 164 (74.9 %) were positive for P. multocida, 45 (20.5 %) for A. pleuropneumoniae and 4 (1.83 %) for H. parasuis. Bacteriological examination revealed that 145 samples (66.2 %) were positive for P. multocida, 31 (14.2 %) for A. pleuropneumoniae and 2 (0.91 %) for H. parasuis.
Obsahuje 2 tabulky
Bibliografie atd.Literatura
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