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Multiplex PCR assay for detection of Actinobacillus pleuropneumoniae, Pasteurella multocida and Haemophilus parasuis in lungs of pigs from a slaughterhouse
M. Hričínová, E. Holoda, D. Mudroňová, S. Ondrašovičová
Folia microbiologica.
2010 ;
55 (6) :
635-640.
Language English Country Czech Republic
Document type Comparative Study
Persistent link
https://www.medvik.cz/link/bmc12003961
- MeSH
- Actinobacillus pleuropneumoniae genetics isolation & purification MeSH
- Bacteriological Techniques methods MeSH
- DNA Primers MeSH
- Financing, Organized MeSH
- Haemophilus parasuis genetics isolation & purification MeSH
- Evaluation Studies as Topic MeSH
- Abattoirs MeSH
- Pasteurella multocida chemistry isolation & purification MeSH
- Lung microbiology MeSH
- Polymerase Chain Reaction methods MeSH
- Swine MeSH
- Prevalence MeSH
- Sensitivity and Specificity MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Comparative Study MeSH
Multiplex PCR has been developed for parallel identification of Actinobacillus pleuropneumoniae, Pasteurella multocida and Haemophilus parasuis, important pathogens of swine, responsible for considerable economic losses in swine industry. Multiplex PCR and bacteriological cultivation was used to analyze lung samples from slaughterhouse pigs. From a total of 219 lung samples, 164 (74.9 %) were positive for P. multocida, 45 (20.5 %) for A. pleuropneumoniae and 4 (1.83 %) for H. parasuis. Bacteriological examination revealed that 145 samples (66.2 %) were positive for P. multocida, 31 (14.2 %) for A. pleuropneumoniae and 2 (0.91 %) for H. parasuis.
Obsahuje 2 tabulky
Bibliography, etc.Literatura
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- $a Multiplex PCR has been developed for parallel identification of Actinobacillus pleuropneumoniae, Pasteurella multocida and Haemophilus parasuis, important pathogens of swine, responsible for considerable economic losses in swine industry. Multiplex PCR and bacteriological cultivation was used to analyze lung samples from slaughterhouse pigs. From a total of 219 lung samples, 164 (74.9 %) were positive for P. multocida, 45 (20.5 %) for A. pleuropneumoniae and 4 (1.83 %) for H. parasuis. Bacteriological examination revealed that 145 samples (66.2 %) were positive for P. multocida, 31 (14.2 %) for A. pleuropneumoniae and 2 (0.91 %) for H. parasuis.
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