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In vitro activation of cytotoxic T-lymphocytes by hTERT-pulsed dendritic cells
Fedor Kryukov, Darina Očadlíková, Lucie Kovářová, Ivana Burešová, Roman Hájek, Jaroslav Michálek
Jazyk angličtina Země Velká Británie
Typ dokumentu práce podpořená grantem
Grantová podpora
1A8709
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Zdroj
E-zdroje NLK Online
Taylor & Francis Open Access od 2004-01-01Medline Complete (EBSCOhost) od 2008-01-01
ROAD: Directory of Open Access Scholarly Resources od 2004
Odkazy
Knihovny.cz E-zdroje
- MeSH
- aktivace lymfocytů MeSH
- antigeny nádorové imunologie MeSH
- cytotoxické T-lymfocyty imunologie MeSH
- dendritické buňky imunologie MeSH
- lidé MeSH
- mnohočetný myelom imunologie MeSH
- nádorové buněčné linie MeSH
- peptidové fragmenty genetika imunologie MeSH
- telomerasa genetika imunologie metabolismus MeSH
- transdukce genetická MeSH
- transfekce MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Multiple myeloma has been considered a weakly immunogenic malignancy that can cause profound defects in the immune system. An important issue for the immunotherapy of myeloma is the identification of appropriate tumor-associated antigens (TAAs). Recently, hTERT (human telomerase reverse transcriptase) was detected on a majority of human malignancies. In the studies reported here, we studied antigen-specific and HLA-A2-restricted cytotoxic activity against an ARH77 myeloma cell line in vitro. An HLA-A2-specific hTERT-derived nonapeptide ((540)ILAKFLHWL(548)) was used as a TAA. Myeloma-specific cytotoxic activity of hTERT-reactive cytotoxic lymphocytes (CTLs) was established by repeated stimulation of the CTLs via dendritic cells loaded with hTERT-derived nonapeptide. These studies were able to demonstrate that hTERT-reactive T-lymphocytes can be identified and expanded using relatively simple in vitro techniques consisting of antigen-specific stimulation, immunomagnetic sorting, and then induction of rapid expansion.
Department of Hemato oncology University Hospital Brno Brno Czech Republic
Department of Pediatrics University Hospital Brno Brno Czech Republic
University Cell Immunotherapy Center Masaryk University Brno Czech Republic
University Research Centre The Czech Myeloma Group Masaryk University Brno Czech Republic
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