-
Something wrong with this record ?
Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP)
Chang-Wang Pan, Ya-Fei Li, Han Wang, Feng Tan
Language English Country Czech Republic
Document type Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1966
ProQuest Central
from 2004-01-01 to 3 months ago
Health & Medicine (ProQuest)
from 2004-01-01 to 3 months ago
Public Health Database (ProQuest)
from 2004-01-01 to 3 months ago
ROAD: Directory of Open Access Scholarly Resources
from 1982
- MeSH
- Humans MeSH
- Mice, Inbred ICR MeSH
- Mice MeSH
- DNA, Protozoan genetics urine MeSH
- Nucleic Acid Amplification Techniques methods MeSH
- Toxoplasma genetics isolation & purification MeSH
- Toxoplasmosis diagnosis parasitology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
In this study, a loop-mediated isothermal amplification (LAMP) assay was established to detect Toxoplasma gondii DNA in mice infected with T. gondii PRU strain. This LAMP assay was based on the sequence of highly repetitive B1 gene. The detection limit of T. gondii LAMP assay was 1 pg of T. gondii DNA, which was evaluated using 10-fold serially diluted DNA of cultured parasites. The LAMP assay was also highly specific for T. gondii and able to detect T. gondii DNA in urine of mice treated with dexamethasone at 90 day post infection (p.i.), although this assay could not detect the DNA in mice urine 2–6 days p.i. These results demonstrated that LAMP is effective for evaluation of therapy effectiveness for T. gondii infection. The established LAMP assay may represent a useful and practical tool for the routine diagnosis and therapeutic evaluation of human toxoplasmosis.
References provided by Crossref.org
Literatura
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12012376
- 003
- CZ-PrNML
- 005
- 20120605143120.0
- 007
- ta
- 008
- 120412s2012 xr o f 000 0eng||
- 009
- AR
- 024 7_
- $a 10.14411/fp.2012.004 $2 doi
- 040 __
- $a ABA008 $d ABA008 $e AACR2 $b cze
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Hu, Xin $u School of Medical Laboratory, Science and School of Life Science, Wenzhou Medical College, Wenzhou, Zhejiang 325035, P. R. China
- 245 10
- $a Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP) / $c Chang-Wang Pan, Ya-Fei Li, Han Wang, Feng Tan
- 504 __
- $a Literatura $b 35
- 520 9_
- $a In this study, a loop-mediated isothermal amplification (LAMP) assay was established to detect Toxoplasma gondii DNA in mice infected with T. gondii PRU strain. This LAMP assay was based on the sequence of highly repetitive B1 gene. The detection limit of T. gondii LAMP assay was 1 pg of T. gondii DNA, which was evaluated using 10-fold serially diluted DNA of cultured parasites. The LAMP assay was also highly specific for T. gondii and able to detect T. gondii DNA in urine of mice treated with dexamethasone at 90 day post infection (p.i.), although this assay could not detect the DNA in mice urine 2–6 days p.i. These results demonstrated that LAMP is effective for evaluation of therapy effectiveness for T. gondii infection. The established LAMP assay may represent a useful and practical tool for the routine diagnosis and therapeutic evaluation of human toxoplasmosis.
- 650 02
- $a zvířata $7 D000818
- 650 02
- $a protozoální DNA $x genetika $x moč $7 D016054
- 650 02
- $a ženské pohlaví $7 D005260
- 650 02
- $a lidé $7 D006801
- 650 02
- $a myši $7 D051379
- 650 02
- $a myši inbrední ICR $7 D008813
- 650 02
- $a techniky amplifikace nukleových kyselin $x metody $7 D021141
- 650 02
- $a Toxoplasma $x genetika $x izolace a purifikace $7 D014122
- 650 02
- $a toxoplazmóza $x diagnóza $x parazitologie $7 D014123
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Pan, Chang-Wang $u Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical College, Wenzhou, Zhejiang 325035, P. R. China
- 700 1_
- $a Li, Ya-Fei $u School of Medical Laboratory, Science and School of Life Science, Wenzhou Medical College, Wenzhou, Zhejiang 325035, P. R. China
- 700 1_
- $a Wang, Han $u Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical College, Wenzhou, Zhejiang 325035, P. R. China
- 700 1_
- $a Tan, Feng $u Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical College, Wenzhou, Zhejiang 325035, P. R. China
- 773 0_
- $t Folia parasitologica $x 0015-5683 $g Roč. 59, č. 1 (2012), s. 21-26 $w MED00011006
- 856 41
- $u https://folia.paru.cas.cz/pdfs/fol/2012/01/04.pdf $y plný text volně přístupný
- 910 __
- $a ABA008 $b A 2854 $c 558 a $y 2
- 990 __
- $a 20120412073016 $b ABA008
- 991 __
- $a 20120605143046 $b ABA008
- 999 __
- $a ok $b bmc $g 905806 $s 769359
- BAS __
- $a 3
- BMC __
- $a 2012 $b 59 $c 1 $d 21-26 $i 0015-5683 $m Folia parasitologica $n Folia parasitol. $x MED00011006
- LZP __
- $a 2012-22/ipme
