Acinetobacter bohemicus sp. nov. widespread in natural soil and water ecosystems in the Czech Republic
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25074407
DOI
10.1016/j.syapm.2014.07.001
PII: S0723-2020(14)00101-5
Knihovny.cz E-zdroje
- Klíčová slova
- 16S rRNA gene, ANI, Carbon source assimilation, MALDI-TOF MS, gyrB, rpoB,
- MeSH
- Acinetobacter chemie klasifikace genetika izolace a purifikace MeSH
- DNA bakterií chemie genetika MeSH
- DNA gyráza genetika MeSH
- DNA řízené RNA-polymerasy genetika MeSH
- ekosystém MeSH
- fylogeneze MeSH
- histidin metabolismus MeSH
- mikrobiologie vody * MeSH
- molekulární sekvence - údaje MeSH
- půdní mikrobiologie * MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- techniky typizace bakterií MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- DNA bakterií MeSH
- DNA gyráza MeSH
- DNA řízené RNA-polymerasy MeSH
- histidin MeSH
- ribozomální DNA MeSH
- RNA polymerase beta subunit MeSH Prohlížeč
- RNA ribozomální 16S MeSH
We investigated the taxonomic status of a phenetically unique group of 25 Acinetobacter strains which were isolated from multiple soil and water samples collected in natural ecosystems in the Czech Republic. Based on the comparative sequence analyses of the rpoB, gyrB, and 16S rRNA genes, the strains formed a coherent and well separated branch within the genus Acinetobacter. The genomic uniqueness of the group at the species level was supported by the low average nucleotide identity values (≤77.37%) between the whole genome sequences of strain ANC 3994(T) (NCBI accession no. APOH00000000) and the representatives of the known Acinetobacter species. Moreover, all 25 strains created a tight cluster clearly separated from all hitherto described species based on whole-cell protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and shared a unique combination of metabolic and physiological properties. The capacity to assimilate l-histidine and the inability to grow at 35°C differentiated them from their phenotypically closest neighbor, Acinetobacter johnsonii. We conclude that the 25 strains represent a novel Acinetobacter species, for which the name Acinetobacter bohemicus sp. nov. is proposed. The type strain of A. bohemicus is ANC 3994(T) (=CIP 110496(T)=CCUG 63842(T)=CCM 8462(T)).
Citace poskytuje Crossref.org
The genomic diversification of the whole Acinetobacter genus: origins, mechanisms, and consequences
GENBANK
KF679797, KF679798, KF679799, KJ124806, KJ124807, KJ124808, KJ124809, KJ124810, KJ124811, KJ124812, KJ124813, KJ124814, KJ124815, KJ124816, KJ124817, KJ124818, KJ124819, KJ124820, KJ124821, KJ124822, KJ124823, KJ124824, KJ124825, KJ124826, KJ124827, KJ124828, KJ124829, KJ124834, KJ147443, KJ147444, KJ147445, KJ147446, KJ147447, KJ147448, KJ147449, KJ147450, KJ147451, KJ147452, KJ147453, KJ147454, KJ147455, KJ147456, KJ147457, KJ147458, KJ147459, KJ147460, KJ147461, KJ147462, KJ147463, KJ147464, KJ147465, KJ147466, KJ147467
