Acinetobacter bohemicus sp. nov. widespread in natural soil and water ecosystems in the Czech Republic
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25074407
DOI
10.1016/j.syapm.2014.07.001
PII: S0723-2020(14)00101-5
Knihovny.cz E-resources
- Keywords
- 16S rRNA gene, ANI, Carbon source assimilation, MALDI-TOF MS, gyrB, rpoB,
- MeSH
- Acinetobacter chemistry classification genetics isolation & purification MeSH
- DNA, Bacterial chemistry genetics MeSH
- DNA Gyrase genetics MeSH
- DNA-Directed RNA Polymerases genetics MeSH
- Ecosystem MeSH
- Phylogeny MeSH
- Histidine metabolism MeSH
- Water Microbiology * MeSH
- Molecular Sequence Data MeSH
- Soil Microbiology * MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Bacterial Typing Techniques MeSH
- Temperature MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- DNA, Bacterial MeSH
- DNA Gyrase MeSH
- DNA-Directed RNA Polymerases MeSH
- Histidine MeSH
- DNA, Ribosomal MeSH
- RNA polymerase beta subunit MeSH Browser
- RNA, Ribosomal, 16S MeSH
We investigated the taxonomic status of a phenetically unique group of 25 Acinetobacter strains which were isolated from multiple soil and water samples collected in natural ecosystems in the Czech Republic. Based on the comparative sequence analyses of the rpoB, gyrB, and 16S rRNA genes, the strains formed a coherent and well separated branch within the genus Acinetobacter. The genomic uniqueness of the group at the species level was supported by the low average nucleotide identity values (≤77.37%) between the whole genome sequences of strain ANC 3994(T) (NCBI accession no. APOH00000000) and the representatives of the known Acinetobacter species. Moreover, all 25 strains created a tight cluster clearly separated from all hitherto described species based on whole-cell protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and shared a unique combination of metabolic and physiological properties. The capacity to assimilate l-histidine and the inability to grow at 35°C differentiated them from their phenotypically closest neighbor, Acinetobacter johnsonii. We conclude that the 25 strains represent a novel Acinetobacter species, for which the name Acinetobacter bohemicus sp. nov. is proposed. The type strain of A. bohemicus is ANC 3994(T) (=CIP 110496(T)=CCUG 63842(T)=CCM 8462(T)).
References provided by Crossref.org
The genomic diversification of the whole Acinetobacter genus: origins, mechanisms, and consequences
GENBANK
KF679797, KF679798, KF679799, KJ124806, KJ124807, KJ124808, KJ124809, KJ124810, KJ124811, KJ124812, KJ124813, KJ124814, KJ124815, KJ124816, KJ124817, KJ124818, KJ124819, KJ124820, KJ124821, KJ124822, KJ124823, KJ124824, KJ124825, KJ124826, KJ124827, KJ124828, KJ124829, KJ124834, KJ147443, KJ147444, KJ147445, KJ147446, KJ147447, KJ147448, KJ147449, KJ147450, KJ147451, KJ147452, KJ147453, KJ147454, KJ147455, KJ147456, KJ147457, KJ147458, KJ147459, KJ147460, KJ147461, KJ147462, KJ147463, KJ147464, KJ147465, KJ147466, KJ147467
