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Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP)
Chang-Wang Pan, Ya-Fei Li, Han Wang, Feng Tan
Jazyk angličtina Země Česko
Typ dokumentu práce podpořená grantem
NLK
Free Medical Journals
od 1966
ProQuest Central
od 2004-01-01 do Před 3 měsíci
Health & Medicine (ProQuest)
od 2004-01-01 do Před 3 měsíci
Public Health Database (ProQuest)
od 2004-01-01 do Před 3 měsíci
ROAD: Directory of Open Access Scholarly Resources
od 1982
- MeSH
- lidé MeSH
- myši inbrední ICR MeSH
- myši MeSH
- protozoální DNA genetika moč MeSH
- techniky amplifikace nukleových kyselin metody MeSH
- Toxoplasma genetika izolace a purifikace MeSH
- toxoplazmóza diagnóza parazitologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
In this study, a loop-mediated isothermal amplification (LAMP) assay was established to detect Toxoplasma gondii DNA in mice infected with T. gondii PRU strain. This LAMP assay was based on the sequence of highly repetitive B1 gene. The detection limit of T. gondii LAMP assay was 1 pg of T. gondii DNA, which was evaluated using 10-fold serially diluted DNA of cultured parasites. The LAMP assay was also highly specific for T. gondii and able to detect T. gondii DNA in urine of mice treated with dexamethasone at 90 day post infection (p.i.), although this assay could not detect the DNA in mice urine 2–6 days p.i. These results demonstrated that LAMP is effective for evaluation of therapy effectiveness for T. gondii infection. The established LAMP assay may represent a useful and practical tool for the routine diagnosis and therapeutic evaluation of human toxoplasmosis.
Citace poskytuje Crossref.org
Literatura
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- $a In this study, a loop-mediated isothermal amplification (LAMP) assay was established to detect Toxoplasma gondii DNA in mice infected with T. gondii PRU strain. This LAMP assay was based on the sequence of highly repetitive B1 gene. The detection limit of T. gondii LAMP assay was 1 pg of T. gondii DNA, which was evaluated using 10-fold serially diluted DNA of cultured parasites. The LAMP assay was also highly specific for T. gondii and able to detect T. gondii DNA in urine of mice treated with dexamethasone at 90 day post infection (p.i.), although this assay could not detect the DNA in mice urine 2–6 days p.i. These results demonstrated that LAMP is effective for evaluation of therapy effectiveness for T. gondii infection. The established LAMP assay may represent a useful and practical tool for the routine diagnosis and therapeutic evaluation of human toxoplasmosis.
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