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Highly superporous cholesterol-modified poly(2-hydroxyethyl methacrylate) scaffolds for spinal cord injury repair
S. Kubinová, D. Horák, A. Hejčl, Z. Plichta, J. Kotek, E. Syková
Language English Country United States
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
21953978
DOI
10.1002/jbm.a.33221
Knihovny.cz E-resources
- MeSH
- Biocompatible Materials chemistry MeSH
- Cell Adhesion MeSH
- Cholesterol chemistry MeSH
- Implants, Experimental MeSH
- Hydrogels chemistry MeSH
- Rats MeSH
- Cells, Cultured MeSH
- Stress, Mechanical MeSH
- Methacrylates chemistry MeSH
- Mesenchymal Stem Cells cytology physiology MeSH
- Molecular Structure MeSH
- Polyhydroxyethyl Methacrylate chemistry MeSH
- Spinal Cord Injuries pathology MeSH
- Porosity MeSH
- Rats, Wistar MeSH
- Cell Proliferation MeSH
- Spinal Cord Regeneration MeSH
- Nerve Regeneration MeSH
- Materials Testing MeSH
- Tissue Scaffolds chemistry MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
Modifications of poly(2-hydroxyethyl methacrylate) (PHEMA) with cholesterol and the introduction of large pores have been developed to create highly superporous hydrogels that promote cell-surface interactions and that can serve as a permissive scaffold for spinal cord injury (SCI) treatment. Highly superporous cholesterol-modified PHEMA scaffolds have been prepared by the bulk radical copolymerization of 2-hydroxyethyl methacrylate (HEMA), cholesterol methacrylate (CHLMA), and ethylene dimethacrylate (EDMA) cross-linking agent in the presence of ammonium oxalate crystals to establish interconnected pores in the scaffold. Moreover, 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA) was incorporated in the polymerization recipe and hydrolyzed, thus introducing carboxyl groups in the hydrogel to control its swelling and softness. The hydrogels supported the in vitro adhesion and proliferation of rat mesenchymal stem cells. In an in vivo study of acute rat SCI, hydrogels were implanted to bridge a hemisection cavity. Histological evaluation was done 4 weeks after implantation and revealed the good incorporation of the implanted hydrogels into the surrounding tissue, the progressive infiltration of connective tissue and the ingrowth of neurofilaments, Schwann cells, and blood vessels into the hydrogel pores. The results show that highly superporous cholesterol-modified PHEMA hydrogels have bioadhesive properties and are able to bridge a spinal cord lesion.
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