-
Something wrong with this record ?
Distinguishing of primary mediastinal B-cell lymphoma and diffuse large B-cell lymphoma using real-time quantitative polymerase chain reaction
H. Votavova, K. Forsterova, V. Campr, J. Sritesky, Z. Velenska, R. Pytlik, K. Kubackova, B. Prochazka, R. Kodet, I. Spicka, H. Krejcova, M. Trneny, P. Klener,
Language English Country Slovakia
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NS9791
MZ0
CEP Register
- MeSH
- Lymphoma, B-Cell diagnosis MeSH
- Diagnosis, Differential MeSH
- Lymphoma, Large B-Cell, Diffuse diagnosis MeSH
- Adult MeSH
- Immunohistochemistry MeSH
- Middle Aged MeSH
- Humans MeSH
- Mediastinal Neoplasms diagnosis MeSH
- Polymerase Chain Reaction methods MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Primary mediastinal B-cell lymphoma (PMBL) seems to be reliably distinguished from diffuse large B-cell lymphoma (DLBCL) with microarray technology. We measured expression of Fcer2, Pdl2 and Blk genes using real-time quantitative polymerase chain reaction (RTqPCR) on formalin fixed, paraffin embedded material (FFPE) and suggested a formula to discriminate PMBL from DLBCL. For 39/82 included patients the diagnosis of PMBL was expected clinico-pathologically. Diagnosis of 10/39 and 2/43 of clinically considered PMBLs and DLBCLs, respectively, was not genetically confirmed. Compared to confirmed PMBLs, unconfirmed ones showed clinical features similar to DLBCLs, e.g. spleen infiltration (p=0,028) and decreased invasiveness in pericardium (p=0,045). They tended to have more common infradiaphragmatic involvement, less often tumor sclerosis or fluidothorax. There were no immunohistochemical differences between genetically confirmed and unconfirmed PMBLs. New approach of distinguishing PMBL and DLBCL is presented. It is based on expression of three genes in routinely available FFPE material using RTqPCR.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc12026320
- 003
- CZ-PrNML
- 005
- 20170411101651.0
- 007
- ta
- 008
- 120817s2010 xo f 000 0#eng||
- 009
- AR
- 024 7_
- $a 10.4149/neo_2010_05_449 $2 doi
- 035 __
- $a (PubMed)20568899
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xo
- 100 1_
- $a Votavova, H $u Charles University, Prague, Czech Republic. tafka@seznam.cz
- 245 10
- $a Distinguishing of primary mediastinal B-cell lymphoma and diffuse large B-cell lymphoma using real-time quantitative polymerase chain reaction / $c H. Votavova, K. Forsterova, V. Campr, J. Sritesky, Z. Velenska, R. Pytlik, K. Kubackova, B. Prochazka, R. Kodet, I. Spicka, H. Krejcova, M. Trneny, P. Klener,
- 520 9_
- $a Primary mediastinal B-cell lymphoma (PMBL) seems to be reliably distinguished from diffuse large B-cell lymphoma (DLBCL) with microarray technology. We measured expression of Fcer2, Pdl2 and Blk genes using real-time quantitative polymerase chain reaction (RTqPCR) on formalin fixed, paraffin embedded material (FFPE) and suggested a formula to discriminate PMBL from DLBCL. For 39/82 included patients the diagnosis of PMBL was expected clinico-pathologically. Diagnosis of 10/39 and 2/43 of clinically considered PMBLs and DLBCLs, respectively, was not genetically confirmed. Compared to confirmed PMBLs, unconfirmed ones showed clinical features similar to DLBCLs, e.g. spleen infiltration (p=0,028) and decreased invasiveness in pericardium (p=0,045). They tended to have more common infradiaphragmatic involvement, less often tumor sclerosis or fluidothorax. There were no immunohistochemical differences between genetically confirmed and unconfirmed PMBLs. New approach of distinguishing PMBL and DLBCL is presented. It is based on expression of three genes in routinely available FFPE material using RTqPCR.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a diferenciální diagnóza $7 D003937
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a imunohistochemie $7 D007150
- 650 _2
- $a B-buněčný lymfom $x diagnóza $7 D016393
- 650 _2
- $a difúzní velkobuněčný B-lymfom $x diagnóza $7 D016403
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a nádory mediastina $x diagnóza $7 D008479
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a polymerázová řetězová reakce $x metody $7 D016133
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Forsterová, Kristina $7 xx0209605
- 700 1_
- $a Campr, V
- 700 1_
- $a Sritesky, J
- 700 1_
- $a Velenska, Z
- 700 1_
- $a Pytlik, R
- 700 1_
- $a Kubackova, K
- 700 1_
- $a Prochazka, B
- 700 1_
- $a Kodet, R
- 700 1_
- $a Spicka, I
- 700 1_
- $a Krejcova, H
- 700 1_
- $a Trneny, M
- 700 1_
- $a Klener, P
- 773 0_
- $w MED00003470 $t Neoplasma $x 0028-2685 $g Roč. 57, č. 5 (2010), s. 449-454
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/20568899 $y Pubmed
- 910 __
- $a ABA008 $b A 1194 $c 659 $y m $z 0
- 990 __
- $a 20120817 $b ABA008
- 991 __
- $a 20170411101949 $b ABA008
- 999 __
- $a ok $b bmc $g 948362 $s 783666
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2010 $b 57 $c 5 $d 449-454 $i 0028-2685 $m Neoplasma $n Neoplasma $x MED00003470
- GRA __
- $a NS9791 $p MZ0
- LZP __
- $a Pubmed-20120817/10/04
