Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins

P. Tarkowski, K. Floková, K. Václavíková, P. Jaworek, M. Raus, A. Nordström, O. Novák, K. Doležal, M. Sebela, J. Frébortová

. 2010 ; 15 (12) : 9214-9229. [pub] 20101215

Language English Country Switzerland

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc12026592

An improved method for determining the relative biosynthetic rate of isoprenoid cytokinins has been developed. A set of 11 relevant isoprenoid cytokinins, including zeatin isomers, was separated by ultra performance liquid chromatography in less than 6 min. The iP-type cytokinins were observed to give rise to a previously-unknown fragment at m/z 69; we suggest that the diagnostic (204-69) transition can be used to monitor the biosynthetic rate of isopentenyladenine. Furthermore, we found that by treating the cytokinin nucleotides with alkaline phosphatase prior to analysis, the sensitivity of the detection process could be increased. In addition, derivatization (propionylation) improved the ESI-MS response by increasing the analytes' hydrophobicity. Indeed, the ESI-MS response of propionylated isopentenyladenosine was about 34% higher than that of its underivatized counterpart. Moreover, the response of the derivatized zeatin ribosides was about 75% higher than that of underivatized zeatin ribosides. Finally, we created a web-based calculator (IZOTOP) that facilitates MS/MS data processing and offer it freely to the research community.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12026592
003      
CZ-PrNML
005      
20160228092924.0
007      
ta
008      
120816s2010 sz f 000 0#eng||
009      
AR
024    7_
$a 10.3390/molecules15129214 $2 doi
035    __
$a (PubMed)21160450
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Tarkowski, Petr, $d 1976- $7 xx0119830 $u Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 11, 78371 Olomouc, Czech Republic. petr.tarkowski@upol.cz
245    13
$a An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins / $c P. Tarkowski, K. Floková, K. Václavíková, P. Jaworek, M. Raus, A. Nordström, O. Novák, K. Doležal, M. Sebela, J. Frébortová
520    9_
$a An improved method for determining the relative biosynthetic rate of isoprenoid cytokinins has been developed. A set of 11 relevant isoprenoid cytokinins, including zeatin isomers, was separated by ultra performance liquid chromatography in less than 6 min. The iP-type cytokinins were observed to give rise to a previously-unknown fragment at m/z 69; we suggest that the diagnostic (204-69) transition can be used to monitor the biosynthetic rate of isopentenyladenine. Furthermore, we found that by treating the cytokinin nucleotides with alkaline phosphatase prior to analysis, the sensitivity of the detection process could be increased. In addition, derivatization (propionylation) improved the ESI-MS response by increasing the analytes' hydrophobicity. Indeed, the ESI-MS response of propionylated isopentenyladenosine was about 34% higher than that of its underivatized counterpart. Moreover, the response of the derivatized zeatin ribosides was about 75% higher than that of underivatized zeatin ribosides. Finally, we created a web-based calculator (IZOTOP) that facilitates MS/MS data processing and offer it freely to the research community.
650    _2
$a Arabidopsis $x metabolismus $7 D017360
650    _2
$a cytokininy $x biosyntéza $x chemie $7 D003583
650    _2
$a deuterium $x chemie $x metabolismus $x farmakologie $7 D003903
650    _2
$a internet $7 D020407
650    _2
$a izotopové značení $x metody $7 D007553
650    _2
$a software $7 D012984
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Floková, Kristýna $7 _AN069092 $u Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR & Palacký University, Šlechtitelů 11, CZ-78371 Olomouc, Czech Republic
700    1_
$a Václavíková, Kateřina
700    1_
$a Jaworek, Pavel
700    1_
$a Raus, Martin $7 _AN072653
700    1_
$a Nordström, Anders $u Umeå Plant Science Centre, Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 901 83 Umeå, Sweden
700    1_
$a Novák, Ondřej $7 xx0120183 $u Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR & Palacký University, Šlechtitelů 11, CZ-78371 Olomouc, Czech Republic. Electronic address: novako@ueb.cas.cz
700    1_
$a Doležal, Karel, $d 1967- $7 xx0100056 $u Laboratory of Growth Regulators, Palacky University & Institute of Experimental Botany AS CR, Slechtitelů 11, 783 71 Olomouc, Czech Republic. dolezal@risc.upol.cz
700    1_
$a Šebela, Marek, $d 1971- $7 jo20000082920 $u Department of Biochemistry, Faculty of Science, Palacký University, Slechtitelů 11, CZ-783 71 Olomouc, Czech Republic. marek.sebela@upol.cz
700    1_
$a Frébortová, Jitka, $d 1968- $7 mzk2009492219 $u Laboratory of Growth Regulators, Faculty of Science, Palacký University/Institute of Experimental Botany of the Academy of Science, Slechtitelů 11, 783 71 Olomouc, Czech Republic
773    0_
$w MED00180394 $t Molecules (Basel, Switzerland) $x 1420-3049 $g Roč. 15, č. 12 (2010), s. 9214-9229
856    41
$u https://pubmed.ncbi.nlm.nih.gov/21160450 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y m $z 0
990    __
$a 20120816 $b ABA008
991    __
$a 20160228092934 $b ABA008
999    __
$a ok $b bmc $g 948634 $s 783938
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2010 $b 15 $c 12 $d 9214-9229 $e 20101215 $i 1420-3049 $m Molecules $n Molecules $x MED00180394
LZP    __
$b NLK122 $a Pubmed-20120816/11/01
Back

Find record

Citation metrics

Archiving options