• Something wrong with this record ?

Identification of Staphylococcus spp. using (GTG)₅-PCR fingerprinting

P. Svec, R. Pantůček, P. Petráš, I. Sedláček, D. Nováková

. 2010 ; 33 (8) : 451-456. [pub] 20101120

Language English Country Germany

Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc12026626

A group of 212 type and reference strains deposited in the Czech Collection of Microorganisms (Brno, Czech Republic) and covering 41 Staphylococcus species comprising 21 subspecies was characterised using rep-PCR fingerprinting with the (GTG)₅ primer in order to evaluate this method for identification of staphylococci. All strains were typeable using the (GTG)₅ primer and generated PCR products ranging from 200 to 4500 bp. Numerical analysis of the obtained fingerprints revealed (sub)species-specific clustering corresponding with the taxonomic position of analysed strains. Taxonomic position of selected strains representing the (sub)species that were distributed over multiple rep-PCR clusters was verified and confirmed by the partial rpoB gene sequencing. Staphylococcus caprae, Staphylococcus equorum, Staphylococcus sciuri, Staphylococcus piscifermentans, Staphylococcus xylosus, and Staphylococcus saprophyticus revealed heterogeneous fingerprints and each (sub)species was distributed over several clusters. However, representatives of the remaining Staphylococcus spp. were clearly separated in single (sub)species-specific clusters. These results showed rep-PCR with the (GTG)₅ primer as a fast and reliable method applicable for differentiation and straightforward identification of majority of Staphylococcus spp.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12026626
003      
CZ-PrNML
005      
20160301085255.0
007      
ta
008      
120816s2010 gw f 000 0#eng||
009      
AR
024    7_
$a 10.1016/j.syapm.2010.09.004 $2 doi
035    __
$a (PubMed)21095086
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Švec, Pavel $7 xx0050849 $u Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic. mpavel@sci.muni.cz
245    10
$a Identification of Staphylococcus spp. using (GTG)₅-PCR fingerprinting / $c P. Svec, R. Pantůček, P. Petráš, I. Sedláček, D. Nováková
520    9_
$a A group of 212 type and reference strains deposited in the Czech Collection of Microorganisms (Brno, Czech Republic) and covering 41 Staphylococcus species comprising 21 subspecies was characterised using rep-PCR fingerprinting with the (GTG)₅ primer in order to evaluate this method for identification of staphylococci. All strains were typeable using the (GTG)₅ primer and generated PCR products ranging from 200 to 4500 bp. Numerical analysis of the obtained fingerprints revealed (sub)species-specific clustering corresponding with the taxonomic position of analysed strains. Taxonomic position of selected strains representing the (sub)species that were distributed over multiple rep-PCR clusters was verified and confirmed by the partial rpoB gene sequencing. Staphylococcus caprae, Staphylococcus equorum, Staphylococcus sciuri, Staphylococcus piscifermentans, Staphylococcus xylosus, and Staphylococcus saprophyticus revealed heterogeneous fingerprints and each (sub)species was distributed over several clusters. However, representatives of the remaining Staphylococcus spp. were clearly separated in single (sub)species-specific clusters. These results showed rep-PCR with the (GTG)₅ primer as a fast and reliable method applicable for differentiation and straightforward identification of majority of Staphylococcus spp.
650    _2
$a zvířata $7 D000818
650    _2
$a shluková analýza $7 D016000
650    _2
$a DNA fingerprinting $x metody $7 D016172
650    _2
$a DNA primery $x genetika $7 D017931
650    _2
$a DNA bakterií $x genetika $7 D004269
650    _2
$a DNA řízené RNA-polymerasy $x chemie $7 D012321
650    _2
$a lidé $7 D006801
650    _2
$a molekulární sekvence - údaje $7 D008969
650    _2
$a fylogeneze $7 D010802
650    _2
$a polymerázová řetězová reakce $x metody $7 D016133
650    _2
$a podjednotky proteinů $x genetika $7 D021122
650    _2
$a stafylokokové infekce $x mikrobiologie $7 D013203
650    _2
$a Staphylococcus $x klasifikace $x genetika $x izolace a purifikace $7 D013210
655    _2
$a hodnotící studie $7 D023362
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Pantůček, Roman, $d 1969- $7 uzp2006324651 $u Division of Genetics and Molecular Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic
700    1_
$a Petráš, Petr, $d 1942- $7 nlk20020117485 $u Reference Laboratory for Staphylococci, National Institute of Public Health, Šrobárova 48, 100 42, Praha 10, Czech Republic
700    1_
$a Sedláček, Ivo, $d 1958- $7 jx20040810004 $u Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic
700    1_
$a Nováková, Dana $7 xx0124249 $u Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic
773    0_
$w MED00004831 $t Systematic and applied microbiology $x 1618-0984 $g Roč. 33, č. 8 (2010), s. 451-456
856    41
$u https://pubmed.ncbi.nlm.nih.gov/21095086 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y m $z 0
990    __
$a 20120816 $b ABA008
991    __
$a 20160301085308 $b ABA008
999    __
$a ok $b bmc $g 948668 $s 783972
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2010 $b 33 $c 8 $d 451-456 $e 20101120 $i 1618-0984 $m Systematic and applied microbiology $n Syst Appl Microbiol $x MED00004831
LZP    __
$b NLK122 $a Pubmed-20120816/11/01

Find record

Citation metrics

Archiving options