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Detection of bisphenol A using a novel surface plasmon resonance biosensor
K. Hegnerová, M. Piliarik, M. Šteinbachová, Z. Flegelová, H. Černohorská, J. Homola,
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biosensing Techniques methods MeSH
- Water Pollutants, Chemical analysis chemistry MeSH
- Phenols analysis chemistry MeSH
- Limit of Detection MeSH
- Surface Plasmon Resonance methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
We present a compact surface plasmon resonance (SPR) biosensor for the detection of bisphenol A (BpA), an endocrine-disrupting chemical. The biosensor is based on an SPR sensor platform (SPRCD) and the binding inhibition detection format. The detection of BpA in PBS and wastewater was performed at concentrations ranging from 0.05 to 1,000 ng/ml. The limit of detection for BpA in PBS and wastewater was estimated to be 0.08 and 0.14 ng/ml, respectively. It was also demonstrated that the biosensor can be regenerated for repeated use. Results achieved with the SPR biosensor are compared with those obtained using ELISA and HPLC methods.
References provided by Crossref.org
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