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Effect of cytoskeleton inhibitors on conidiogenesis and capsule in the long neck yeast Fellomyces examined by scanning electron microscopy
M. Kopecká, L. Ilkovics, V. Ramíková, M. Yamaguchi,
Language English Country Switzerland
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 1998-01-01 to 2015-11-30
Health & Medicine (ProQuest)
from 1998-01-01 to 2015-11-30
PubMed
20551635
DOI
10.1159/000316330
Knihovny.cz E-resources
- MeSH
- Basidiomycota drug effects ultrastructure MeSH
- Bridged Bicyclo Compounds, Heterocyclic pharmacology MeSH
- Cytoskeleton drug effects ultrastructure MeSH
- Microscopy, Electron, Scanning methods MeSH
- Spores, Fungal drug effects ultrastructure MeSH
- Thiazolidines pharmacology MeSH
- Cell Shape drug effects MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
BACKGROUND: The aim of this basic study was to investigate by scanning electron microscopy the effects of cytoskeleton inhibitors on conidiogenesis and capsule in the yeast Fellomyces fuzhouensis CBS 8243, related to Cryptococcus neoformans. METHODS: Cells were treated by methyl benzimidazole-2-ylcarbamate (BCM) and latrunculin A (LAT) in yeast extract peptone dextrose medium and examined by scanning electron microscopy. RESULTS: During conidiogenesis, mother cells covered by capsule formed hypha-like stalks and at the hyphal tip yeast-like conidium developed. LAT blocked both stages of conidiogenesis. Inhibited mother cells and conidia became spherical and their capsule disappeared. BCM did not block formation of conidia that were neckless, or affect capsule. Combined application of LAT and BCM blocked both stages of conidiogenesis, cells became spherical and their capsule disappeared. CONCLUSION: Yeast cells with disrupted actin cytoskeleton do not reproduce by conidiogenesis and do not retain inherited cell shape and capsule.
References provided by Crossref.org
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