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Investigation of ornamental fish entering the EU for the presence of ranaviruses
T. Vesely, K. Cinkova, S. Reschova, F. Gobbo, E. Ariel, M. Vicenova, D. Pokorova, P. Kulich, G. Bovo
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- buněčné linie virologie MeSH
- DNA-dependentní DNA-polymerasy analýza genetika MeSH
- Evropská unie MeSH
- fylogeneze MeSH
- infekce DNA virem genetika veterinární MeSH
- karcinom virologie MeSH
- nemoci ryb virologie MeSH
- polymerázová řetězová reakce MeSH
- Ranavirus klasifikace enzymologie genetika ultrastruktura MeSH
- ryby virologie MeSH
- transmisní elektronová mikroskopie MeSH
- virové plášťové proteiny analýza genetika MeSH
- virové proteiny analýza genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A survey was performed on ornamental fish imported into the EU to detect viral agents belonging to the genus Ranavirus. The objective was to gain knowledge of the potential for these systemic iridoviruses to gain entry into the EU via international trade in ornamental fish. A total of 208 pooled samples, representing 753 individual fish, were tested. The samples included 13 orders and 37 families, originating from different countries and continents. Tissues from fish that died during or just after transport were collected and examined by standard virological techniques in epithelioma papulosum cyprini cells, by transmission electron microscopy and by PCR for the detection of the major capsid protein and DNA polymerase gene sequences of ranaviruses. Virus was isolated from nine fish species but ranavirus was not identified in those samples. The results suggest that ranaviruses are not highly prevalent in ornamental fish imported into the EU.
Istituto Zooprofilattico Sperimentale delle Venezie Padova Italy
School of Veterinary and Biomedical Sciences James Cook University Townsville Qld Australia
Citace poskytuje Crossref.org
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- $a A survey was performed on ornamental fish imported into the EU to detect viral agents belonging to the genus Ranavirus. The objective was to gain knowledge of the potential for these systemic iridoviruses to gain entry into the EU via international trade in ornamental fish. A total of 208 pooled samples, representing 753 individual fish, were tested. The samples included 13 orders and 37 families, originating from different countries and continents. Tissues from fish that died during or just after transport were collected and examined by standard virological techniques in epithelioma papulosum cyprini cells, by transmission electron microscopy and by PCR for the detection of the major capsid protein and DNA polymerase gene sequences of ranaviruses. Virus was isolated from nine fish species but ranavirus was not identified in those samples. The results suggest that ranaviruses are not highly prevalent in ornamental fish imported into the EU.
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