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Investigation of ornamental fish entering the EU for the presence of ranaviruses
T. Vesely, K. Cinkova, S. Reschova, F. Gobbo, E. Ariel, M. Vicenova, D. Pokorova, P. Kulich, G. Bovo
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cell Line virology MeSH
- DNA-Directed DNA Polymerase analysis genetics MeSH
- European Union MeSH
- Phylogeny MeSH
- DNA Virus Infections genetics veterinary MeSH
- Carcinoma virology MeSH
- Fish Diseases virology MeSH
- Polymerase Chain Reaction MeSH
- Ranavirus classification enzymology genetics ultrastructure MeSH
- Fishes virology MeSH
- Microscopy, Electron, Transmission MeSH
- Capsid Proteins analysis genetics MeSH
- Viral Proteins analysis genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
A survey was performed on ornamental fish imported into the EU to detect viral agents belonging to the genus Ranavirus. The objective was to gain knowledge of the potential for these systemic iridoviruses to gain entry into the EU via international trade in ornamental fish. A total of 208 pooled samples, representing 753 individual fish, were tested. The samples included 13 orders and 37 families, originating from different countries and continents. Tissues from fish that died during or just after transport were collected and examined by standard virological techniques in epithelioma papulosum cyprini cells, by transmission electron microscopy and by PCR for the detection of the major capsid protein and DNA polymerase gene sequences of ranaviruses. Virus was isolated from nine fish species but ranavirus was not identified in those samples. The results suggest that ranaviruses are not highly prevalent in ornamental fish imported into the EU.
Istituto Zooprofilattico Sperimentale delle Venezie Padova Italy
School of Veterinary and Biomedical Sciences James Cook University Townsville Qld Australia
References provided by Crossref.org
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- $a A survey was performed on ornamental fish imported into the EU to detect viral agents belonging to the genus Ranavirus. The objective was to gain knowledge of the potential for these systemic iridoviruses to gain entry into the EU via international trade in ornamental fish. A total of 208 pooled samples, representing 753 individual fish, were tested. The samples included 13 orders and 37 families, originating from different countries and continents. Tissues from fish that died during or just after transport were collected and examined by standard virological techniques in epithelioma papulosum cyprini cells, by transmission electron microscopy and by PCR for the detection of the major capsid protein and DNA polymerase gene sequences of ranaviruses. Virus was isolated from nine fish species but ranavirus was not identified in those samples. The results suggest that ranaviruses are not highly prevalent in ornamental fish imported into the EU.
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