Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

The spectrum of cytokeratins expressed in the adult human cornea, limbus and perilimbal conjunctiva

S. Merjava, A. Neuwirth, M. Tanzerova, K. Jirsova

. 2011 ; 26 (3) : 323-331.

Jazyk angličtina Země Španělsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc12027151

The aim of this study was to detect a spectrum of cytokeratins (CK) present in the adult human cornea, limbus and perilimbal conjunctiva. Cryosections from seven corneo-scleral discs were fixed, and indirect immunofluorescent staining was performed using antibodies directed against CK1-CK10 and CK13-CK20. The percentage of positive cells was calculated in the epithelium of the cornea, limbus and perilimbal conjunctiva. Quantitative real time RT-PCR (qRT-PCR) was used to detect CK6 and CK18 expression in the corneal and conjunctival epithelium. The most intense staining present throughout the cornea was observed for CK3, CK5 and CK14; CK19 was found at the corneal periphery only. CK4 and CK10/13 revealed mild to moderate positivity mostly in the superficial layers of the cornea. The suprabasal cell layers of all examined areas showed a strong positivity for CK16. A heterogeneous staining pattern with a centrifugal decrease in the signal was observed for CK8 and CK18. CK5/6, CK14 and CK19 were present in the limbus, where a positive signal for CK3 was observed in the suprabasal and superficial cells only. In contrast to the cornea, CK15 appeared in the basal and suprabasal layers of the limbus. The perilimbal conjunctiva showed strong immunostaining for CK10/13, CK14 and CK19. A moderate signal for CK7 was detected in the superficial layers of the conjunctiva. qRT-PCR confirmed CK6 and CK18 expression in the corneal and conjunctival epithelium. The detailed characterization of the corneal, limbal and perilimbal conjunctival epithelium under normal circumstances may be useful for characterizing the changes occurring under pathological conditions.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12027151
003      
CZ-PrNML
005      
20230518090820.0
007      
ta
008      
120816s2011 sp f 000 0#eng||
009      
AR
024    7_
$a 10.14670/hh-26.323 $2 doi
035    __
$a (PubMed)21210345
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sp
100    1_
$a Merjava, Stanislava $u Laboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, General Teaching Hospital and Charles University, Prague, Czech Republic
245    14
$a The spectrum of cytokeratins expressed in the adult human cornea, limbus and perilimbal conjunctiva / $c S. Merjava, A. Neuwirth, M. Tanzerova, K. Jirsova
520    9_
$a The aim of this study was to detect a spectrum of cytokeratins (CK) present in the adult human cornea, limbus and perilimbal conjunctiva. Cryosections from seven corneo-scleral discs were fixed, and indirect immunofluorescent staining was performed using antibodies directed against CK1-CK10 and CK13-CK20. The percentage of positive cells was calculated in the epithelium of the cornea, limbus and perilimbal conjunctiva. Quantitative real time RT-PCR (qRT-PCR) was used to detect CK6 and CK18 expression in the corneal and conjunctival epithelium. The most intense staining present throughout the cornea was observed for CK3, CK5 and CK14; CK19 was found at the corneal periphery only. CK4 and CK10/13 revealed mild to moderate positivity mostly in the superficial layers of the cornea. The suprabasal cell layers of all examined areas showed a strong positivity for CK16. A heterogeneous staining pattern with a centrifugal decrease in the signal was observed for CK8 and CK18. CK5/6, CK14 and CK19 were present in the limbus, where a positive signal for CK3 was observed in the suprabasal and superficial cells only. In contrast to the cornea, CK15 appeared in the basal and suprabasal layers of the limbus. The perilimbal conjunctiva showed strong immunostaining for CK10/13, CK14 and CK19. A moderate signal for CK7 was detected in the superficial layers of the conjunctiva. qRT-PCR confirmed CK6 and CK18 expression in the corneal and conjunctival epithelium. The detailed characterization of the corneal, limbal and perilimbal conjunctival epithelium under normal circumstances may be useful for characterizing the changes occurring under pathological conditions.
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    _2
$a konjunktiva $x metabolismus $7 D003228
650    _2
$a rohovka $x metabolismus $7 D003315
650    _2
$a rohovkový epitel $x metabolismus $7 D019573
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a fluorescenční protilátková technika nepřímá $7 D019084
650    _2
$a glyceraldehyd-3-fosfátdehydrogenasy $x metabolismus $7 D005987
650    _2
$a lidé $7 D006801
650    _2
$a imunohistochemie $7 D007150
650    _2
$a keratiny $x biosyntéza $x genetika $x metabolismus $7 D007633
650    _2
$a limbus corneae $x metabolismus $7 D016850
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a fluorescenční mikroskopie $7 D008856
650    _2
$a lidé středního věku $7 D008875
650    _2
$a RNA $x biosyntéza $x genetika $7 D012313
650    _2
$a polymerázová řetězová reakce s reverzní transkripcí $7 D020133
650    _2
$a skléra $x metabolismus $7 D012590
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Neuwirth, Aleš $7 xx0108965 $u Department of Immunobiology, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
700    1_
$a Tanzerová, Michaela $u Laboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, General Teaching Hospital and Charles University, Prague, Czech Republic $7 xx0301706
700    1_
$a Jirsová, Kateřina, $d 1966- $7 xx0101780 $u Laboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, General Teaching Hospital and Charles University, Prague, Czech Republic; Ocular Tissue Bank, General Teaching Hospital and Charles University, Prague, Czech Republic
773    0_
$w MED00005439 $t Histology and histopathology $x 1699-5848 $g Roč. 26, č. 3 (2011), s. 323-331
856    41
$u https://pubmed.ncbi.nlm.nih.gov/21210345 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y m $z 0
990    __
$a 20120816 $b ABA008
991    __
$a 20230518090814 $b ABA008
999    __
$a ok $b bmc $g 949193 $s 784497
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2011 $b 26 $c 3 $d 323-331 $i 1699-5848 $m Histology and histopathology $n Histol Histopathol $x MED00005439
LZP    __
$b NLK112 $a Pubmed-20120816/11/02
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace