• Something wrong with this record ?

In vivo kinetics of U4/U6·U5 tri-snRNP formation in Cajal bodies

I. Novotný, M. Blažíková, D. Staněk, P. Herman, J. Malinsky

. 2011 ; 22 (4) : 513-523.

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc12027190
E-resources Online Full text

NLK Free Medical Journals from 1992 to 2 months ago
PubMed Central from 1992 to 2 months ago
Europe PubMed Central from 1992 to 2 months ago
Open Access Digital Library from 1989-11-01
Open Access Digital Library from 1997-01-01

Links

PubMed 21177826
DOI 10.1091/mbc.e10-07-0560
Knihovny.cz E-resources

The U4/U6·U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) is an essential pre-mRNA splicing factor, which is assembled in a stepwise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs), but little is known about the dynamics of this process. Here we created a mathematical model of tri-snRNP assembly in CBs and used it to fit kinetics of individual snRNPs monitored by fluorescence recovery after photobleaching. A global fitting of all kinetic data determined key reaction constants of tri-snRNP assembly. Our model predicts that the rates of di-snRNP and tri-snRNP assemblies are similar and that ∼230 tri-snRNPs are assembled in one CB per minute. Our analysis further indicates that tri-snRNP assembly is approximately 10-fold faster in CBs than in the surrounding nucleoplasm, which is fully consistent with the importance of CBs for snRNP formation in rapidly developing biological systems. Finally, the model predicted binding between SART3 and a CB component. We tested this prediction by Förster resonance energy transfer and revealed an interaction between SART3 and coilin in CBs.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc12027190
003      
CZ-PrNML
005      
20160408164752.0
007      
ta
008      
120816s2011 xxu f 000 0#eng||
009      
AR
024    7_
$a 10.1091/mbc.e10-07-0560 $2 doi
035    __
$a (PubMed)21177826
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Novotný, Ivan $7 xx0140543 $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic.
245    10
$a In vivo kinetics of U4/U6·U5 tri-snRNP formation in Cajal bodies / $c I. Novotný, M. Blažíková, D. Staněk, P. Herman, J. Malinsky
520    9_
$a The U4/U6·U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) is an essential pre-mRNA splicing factor, which is assembled in a stepwise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs), but little is known about the dynamics of this process. Here we created a mathematical model of tri-snRNP assembly in CBs and used it to fit kinetics of individual snRNPs monitored by fluorescence recovery after photobleaching. A global fitting of all kinetic data determined key reaction constants of tri-snRNP assembly. Our model predicts that the rates of di-snRNP and tri-snRNP assemblies are similar and that ∼230 tri-snRNPs are assembled in one CB per minute. Our analysis further indicates that tri-snRNP assembly is approximately 10-fold faster in CBs than in the surrounding nucleoplasm, which is fully consistent with the importance of CBs for snRNP formation in rapidly developing biological systems. Finally, the model predicted binding between SART3 and a CB component. We tested this prediction by Förster resonance energy transfer and revealed an interaction between SART3 and coilin in CBs.
650    _2
$a antigeny nádorové $x genetika $x metabolismus $7 D000951
650    _2
$a nádorové buněčné linie $7 D045744
650    _2
$a buněčné jádro $x genetika $x metabolismus $7 D002467
650    _2
$a Cajalova tělíska $x genetika $x metabolismus $7 D020541
650    _2
$a HeLa buňky $7 D006367
650    _2
$a lidé $7 D006801
650    _2
$a kinetika $7 D007700
650    _2
$a molekulární modely $7 D008958
650    _2
$a jaderné proteiny $x metabolismus $7 D009687
650    _2
$a vazba proteinů $x genetika $7 D011485
650    _2
$a RNA-helikasy $x genetika $x metabolismus $7 D020365
650    _2
$a prekurzory RNA $x genetika $x metabolismus $7 D012322
650    _2
$a sestřih RNA $x genetika $7 D012326
650    _2
$a proteiny vázající RNA $x genetika $x metabolismus $7 D016601
650    _2
$a malý jaderný ribonukleoprotein U4-U6 $x genetika $x metabolismus $7 D017414
650    _2
$a malý jaderný ribonukleoprotein U5 $x genetika $x metabolismus $7 D017415
650    _2
$a ribonukleoproteiny malé jaderné $x genetika $x metabolismus $7 D017411
650    _2
$a spliceozomy $x genetika $x metabolismus $7 D017381
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Blažíková, Michaela $7 xx0195728 $u Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic; Faculty of Mathematics and Physics, Charles University, 121 16 Prague 2, Czech Republic
700    1_
$a Staněk, David $7 xx0140539 $u Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic
700    1_
$a Herman, Petr $u Faculty of Mathematics and Physics, Charles University, 121 16 Prague 2, Czech Republic
700    1_
$a Malínský, Jan, $d 1970- $7 xx0088621 $u Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic
773    0_
$w MED00005257 $t Molecular biology of the cell $x 1939-4586 $g Roč. 22, č. 4 (2011), s. 513-523
856    41
$u https://pubmed.ncbi.nlm.nih.gov/21177826 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y m $z 0
990    __
$a 20120816 $b ABA008
991    __
$a 20160408164513 $b ABA008
999    __
$a ok $b bmc $g 949232 $s 784536
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2011 $b 22 $c 4 $d 513-523 $i 1939-4586 $m Molecular biology of the cell $n Mol Biol Cell $x MED00005257
LZP    __
$b NLK112 $a Pubmed-20120816/11/02

Find record

Citation metrics

Archiving options